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Chapitre D'ouvrage Année : 2021

Quantitative determination of Andrographolide and related compounds in Andrographis paniculata extracts and biological evaluation of their anti-inflammatory activity

Résumé

Extraction, isolation and characterization of $Andrographis\ paniculata$ (A.p.) products were developed. Three natural diterpenes compounds were obtained and one was used for chemical modifications. Evaluation of their inhibition of TNF$\alpha$ induced NFκB transcriptional activity. A rapid analytical method for the determination and quantitation of three diterpenoid lactones (andrographolide 1, didehydroandrographolide 2, neoandrographiside 3) found in $A.\ paniculata$ extracts was investigated. After some optimizations on column type and injection solvent, the separation was achieved in 9 min on a monolithic Chromolith Performance RP18e column (100 mm × 4.6 mm ID, 2 µm), with a gradient solvent system of water and methanol, UV detection at 220 nm and ELSD detection. The method was proved to be suitable for the quantitation of these three diterpenes in four different commercial $Andrographis$ dietary supplements. The anti-inflammatory activities of a mixture of known composition have been evaluated showing differences in activity depending on the relative ratio of various diterpenes and also a possible synergic activity for some of them.

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hal-03652226 , version 1 (26-04-2022)

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Emmanuelle Villedieu Villedieu-Percheron, Véronique Ferreira, Joana Filomena Campos, Emilie Destandau, Chantal Pichon, et al.. Quantitative determination of Andrographolide and related compounds in Andrographis paniculata extracts and biological evaluation of their anti-inflammatory activity. Proetos, Charlampos. The Benefits of Plant Extracts for Human Health, MDPI Books, pp.129-140, 2021, 978-3-03943-851-8. ⟨10.3390/books978-3-03943-852-5⟩. ⟨hal-03652226⟩
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