Elastic lamellae are important extracellular matrix structures of the arterial wall. During aging and diabetes, they are strongly affected, losing their elasticity, resulting in increased arterial stiffness. Analyzing elastic lamellae geometry and organization is therefore of significance. We took high-resolution synchrotron X-ray microtomography images of mice aorta and segmented elastic lamellae fragments. We used those to measure and estimate the folding of these structures in healthy and diabetic mice. We discuss the choice of sampling parameters to better estimate the real length of elastic lamellae based on skeletonized images. We find that the measurements provide reliable evidence of elastic lamellae alterations due to diabetes.