ADAR-Editing during Ostreid Herpesvirus 1 Infection in Crassostrea gigas : Facts and Limitations - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue MSphere Année : 2022

ADAR-Editing during Ostreid Herpesvirus 1 Infection in Crassostrea gigas : Facts and Limitations

Résumé

Ostreid herpesvirus-1 (OsHV-1) RNAs are enzymatically modified by A-to-I conversions during the infection of Crassostrea gigas. The increase of ADAR1 expression and hyper-editing activity parallel to OsHV-1 RNAs suggests a functional connection between dsRNA editing and antiviral responses. We analyzed 87 RNA-seq data sets from immuno-primed, resistant, and susceptible oysters exposed to OsHV-1 to compare the ADAR hyper-editing levels on host and viral transcripts and trace hyper-editing on the oyster genes. Host RNAs were more hyper-edited than viral RNAs, despite the increased editing of viral RNAs in late infection phases. A set of genes, representing ∼0.5% of the oyster transcriptome and including several tripartite motif-containing sequences, were constantly hyper-edited. Conversely, we identified genes involved in antiviral response, miRNA maturation, and epigenetic regulation that were hyper-edited in specific conditions only. Despite technical and biological bottlenecks that hamper the understanding of the bivalve “RNA editome,” available tools and technologies can be adapted to bivalve mollusks
Fichier principal
Vignette du fichier
Rosani-2022-MSphere-ADAR.pdf (600.34 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03636839 , version 1 (11-04-2022)

Identifiants

Citer

Umberto Rosani, Enrico Bortoletto, C. Montagnani, Paola Venier. ADAR-Editing during Ostreid Herpesvirus 1 Infection in Crassostrea gigas : Facts and Limitations. MSphere, 2022, 7 (2), ⟨10.1128/msphere.00011-22⟩. ⟨hal-03636839⟩
39 Consultations
35 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More