Multicolour-banding fluorescence in situ hybridisation (mbanding-FISH) to identify recurrent chromosomal alterations in breast tumour cell lines - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue British Journal of Cancer Année : 2005

Multicolour-banding fluorescence in situ hybridisation (mbanding-FISH) to identify recurrent chromosomal alterations in breast tumour cell lines

Résumé

Recurrent chromosome breakpoints in tumour cells may point to cancer genes, but not many have been molecularly characterised. We have used multicolour-banding fluorescence in situ hybridisation (mbanding-FISH) on breast tumour cell lines to identify regions of chromosome break created by inversions, duplications, insertions and translocations on chromosomes 1, 5, 8, 12 and 17. We delineate a total of 136 regions of break, some of them occurring with high frequency. We further describe two examples of dual-colour FISH characterisation of breakpoints, which target the 1p36 and 5p11-12 regions. Both breaks involve genes whose function is unknown to date. The mbanding-FISH strategy constitutes an efficient first step in the search for potential cancer genes.

Domaines

Cancer

Dates et versions

hal-03634413 , version 1 (07-04-2022)

Identifiants

Citer

A Letessier, M-J Mozziconacci, A Murati, J Juriens, J Adélaïde, et al.. Multicolour-banding fluorescence in situ hybridisation (mbanding-FISH) to identify recurrent chromosomal alterations in breast tumour cell lines. British Journal of Cancer, 2005, 92 (2), pp.382-388. ⟨10.1038/sj.bjc.6602228⟩. ⟨hal-03634413⟩
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