Format du dépôt |
Fichier |
Type de dépôt |
Article dans une revue |
Titre |
en
Multi-Layered Human Blood Vessels-on-Chip Design Using Double Viscous Finger Patterning
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Résumé |
en
Blood vessel-on-a-chip models aim at reproducing vascular functions. However, very few efficient methods have been designed to address the need for biological replicates in medium- to high-throughput screenings. Here, vessels-on-chip were designed in polydimethylsiloxane-glass chips using the viscous finger patterning technique which was adapted to create channels with various internal diameters inside a collagen solution and to simultaneously seed cells. This method was refined to create blood vessels composed of two concentric, distinct, and closely appositioned layers of human endothelial and perivascular cells arranged around a hollow lumen. These approaches allowed the formation of structurally correct blood vessels-on-chips which were constituted of either only endothelial cells or of both cell types in order to distinguish the vascular barrier reactivity to drugs in the presence or not of perivascular cells. The established vessels showed a tight vascular barrier, as assessed by immunostaining of the adherens junctions, and were reactive to the natural vasopermeant thrombin and to inflammatory cytokines. The presence of perivascular cells markedly increased the tightness of the vascular barrier and lowered its response to thrombin. The design allowed us to simultaneously challenge in real-time several tens of 3D-reconstituted, multicellular blood vessels in a standard multiwell plate format suitable for high-throughput drug screening.
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Auteur(s)
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Elise Delannoy
1, 2, 3
, Géraldine Tellier
3, 4
, Juliette Cholet
3, 4
, Alice Leroy
4, 3
, A. Treizebre
1, 2
, F. Soncin
3, 4
1
IEMN -
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520
( 1066983 )
- [Univ. Lille, CNRS, Centrale Lille Institut, Junia, Univ. Polytechnique Hauts-de-France] ––
Laboratoire Central – Cité Scientifique – Avenue Poincaré – CS 60069 – 59652 VILLENEUVE D’ASCQ CEDEX
- France
-
Centrale Lille ( 120930 )
;
-
Université de Lille ( 374570 )
;
-
Centre National de la Recherche Scientifique UMR8520 ( 441569 )
;
-
Université Polytechnique Hauts-de-France ( 1001263 )
;
-
JUNIA ( 1059196 )
;
-
Université catholique de Lille ( 300877 )
2
BIOMEMS - IEMN -
Bio-Micro-Electro-Mechanical Systems - IEMN
( 1067476 )
- Groupe BioMEMS - IEMN UMR8520
- France
-
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 ( 1066983 )
;
-
Centrale Lille ( 120930 )
;
-
Université de Lille ( 374570 )
;
-
Centre National de la Recherche Scientifique UMR8520 ( 441569 )
;
-
Université Polytechnique Hauts-de-France ( 1001263 )
;
-
JUNIA ( 1059196 )
;
-
Université catholique de Lille ( 300877 )
3
UNICANCER/Lille -
Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille]
( 301886 )
- 3, rue Frédéric Combemale, 59020 Lille
- France
-
Université de Lille ( 374570 )
;
-
UNICANCER ( 502406 )
4
LIMMS -
Laboratory for Integrated Micro Mechatronics Systems
( 2208 )
- LIMMS/CNRS-IIS(UMI 2820)/ Institute of Industrial Science, University of Tokyo/ 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505 Japan/
- Japon
-
The University of Tokyo ( 304304 )
;
-
Centre National de la Recherche Scientifique IRL2820 / UMI2820 ( 441569 )
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Licence |
Paternité
|
Langue du document |
Anglais
|
Vulgarisation |
Non
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Comité de lecture |
Oui
|
Audience |
Internationale
|
Date de publication |
2022-04
|
Volume |
10
|
Numéro |
4
|
Page/Identifiant |
797
|
Nom de la revue |
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Domaine(s) |
-
Sciences de l'ingénieur [physics]
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Collaboration/Projet |
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Financement |
-
This work was supported by grants from Ligue Nationale contre le Cancer (F.S.), Fondation ARC (F.S.), Cancéropôle Nord-Ouest (A.T., F.S.), and by a grant from Contrat de Plan Etat-Région (CPER) Cancer 2015–2020. E.D. salary was supported by the European Union with Fonds Européen de Développement Régional (FEDER)—Région Hauts-de-France (46%), I-SITE ULNE (28%), HCS-Pharma (18%) and IEMN (8%). This work was partly supported by the French Renatech network. F.S. is Director of Research at INSERM.
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Référence interne |
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Mots-clés |
en
blood vessels, organs-on-chips, inflammation, permeability, BioMEMS, microfluidics
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DOI |
10.3390/biomedicines10040797 |