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Article dans une revue Cardiovascular Research Année : 2022

Impacts of a high-fat diet on the metabolic profile and the phenotype of atrial myocardium in mice

Jérôme Piquereau
Maharajah Ponnaiah
Farid Ichou
Camille Pichard
  • Fonction : Auteur
Vincent Galand
  • Fonction : Auteur
Laurence Lucats
  • Fonction : Auteur
Mathias Mericskay

Résumé

Abstract Aims Obesity, diabetes, and metabolic syndromes are risk factors of atrial fibrillation (AF). We tested the hypothesis that metabolic disorders have a direct impact on the atria favouring the formation of the substrate of AF. Methods and results Untargeted metabolomic and lipidomic analysis was used to investigate the consequences of a prolonged high-fat diet (HFD) on mouse atria. Atrial properties were characterized by measuring mitochondria respiration in saponin-permeabilized trabeculae, by recording action potential (AP) with glass microelectrodes in trabeculae and ionic currents in myocytes using the perforated configuration of patch clamp technique and by several immuno-histological and biochemical approaches. After 16 weeks of HFD, obesogenic mice showed a vulnerability to AF. The atrial myocardium acquired an adipogenic and inflammatory phenotypes. Metabolomic and lipidomic analysis revealed a profound transformation of atrial energy metabolism with a predominance of long-chain lipid accumulation and beta-oxidation activation in the obese mice. Mitochondria respiration showed an increased use of palmitoyl-CoA as energy substrate. APs were short duration and sensitive to the K-ATP-dependent channel inhibitor, whereas K-ATP current was enhanced in isolated atrial myocytes of obese mouse. Conclusion HFD transforms energy metabolism, causes fat accumulation, and induces electrical remodelling of the atrial myocardium of mice that become vulnerable to AF.
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hal-03624652, version 1 (30-03-2022)

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Nadine Suffee, Elodie Baptista, Jérôme Piquereau, Maharajah Ponnaiah, Nicolas Doisne, et al.. Impacts of a high-fat diet on the metabolic profile and the phenotype of atrial myocardium in mice. Cardiovascular Research, 2022, 118 (15), pp.3126-3139. ⟨10.1093/cvr/cvab367⟩. ⟨hal-03624652⟩
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