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Article Dans Une Revue New Phytologist Année : 2016

Use of micro RNA ‐encoded peptide mi PEP 172c to stimulate nodulation in soybean

Résumé

Current worldwide agriculture requires huge amounts of chemicals for increasing yields, and this situation will be dramatically degraded by the increasing world population (Velivelli et al., 2014). However, the main problems are that chemicals might be expensive, highly polluting and dangerous for human health, and that some of them, such as phosphates, are available to plants in limited quantities on Earth (Amundson et al., 2015). The search for sustainable alternatives to these chemicals is one of the major challenges of the actual research in plant biology (Oldroyd & Dixon, 2014; Velivelli et al., 2014; Delaux et al., 2015). Another extensively used strategy for improving plant yield is based on conventional breeding methods that require extensive and long-term efforts to identify and introduce favorable traits in crops. While being slightly faster, the use of transgenic methods might nowadays face societal acceptance (Leyser, 2014), but it still needs years of research before its use in agriculture (Oldroyd & Dixon, 2014; Delaux et al., 2015). Another limit of transgenic methods is based on the capacity, and more importantly, the efficiency, of some crops to be regenerated or not. MicroRNAs (miRNAs) are short regulatory RNAs that regulate the expression of their target genes at the post transcriptional level, by cleaving their mRNA or inhibiting their translation. In animals, as in plants, they are involved in most of the developmental processes (Rogers & Chen, 2013). MiRNAs are transcribed by RNA polymerase II as long molecules, called primary transcripts (pri-miRNA). These molecules undergo two successive steps of maturation leading to the formation of the mature, active miRNA. Using miR165a and miR171b in Arabidopsis thaliana and Medicago truncatula, respectively, we have recently shown that these two pri-miRNAs encode for peptides, called miPEPs. These two miPEPs are produced at the same time and location as the primary transcript they come from. MiPEPs increase the quantity of their respective mature miRNA by stimulating the transcription of the associated pri-miRNA (Lauressergues et al., 2015). Finally, it appears that miPEPs are very specific to their associated miRNAs, which might represent a significant advantage in agronomy. MiPEPs have also been shown to modify root development by the external application of synthetic peptides specific to M. truncatula miR171b and A. thaliana miR165a (Lauressergues et al., 2015). These data strongly suggest that miPEPs could be used as tools to increase the agronomical traits of crops (Couzigou et al., 2015).

Dates et versions

hal-03613641 , version 1 (18-03-2022)

Identifiants

Citer

Jean‐malo Couzigou, Olivier André, Bruno Guillotin, Marlène Alexandre, Jean‐philippe Combier. Use of micro RNA ‐encoded peptide mi PEP 172c to stimulate nodulation in soybean. New Phytologist, 2016, 211 (2), pp.379-381. ⟨10.1111/nph.13991⟩. ⟨hal-03613641⟩
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