Detecting the presence and geometry of crustal shear zones by geophysical methods relies on our understanding of the intrinsic parameters controlling the seismic properties of these deformed rocks, over the range of pressuretemperature conditions expected in the Earth's crust. To this end, we aimed to track changes in P-wave propagation velocity (V P), anisotropy (AV P) and S-wave splitting (AV S) across a natural shear zone using experimental and electron backscatter diffraction methods. Five samples were collected across a meter-scale shear zone developed under amphibolite-to greenschist-facies in the Neves metagranodiorite (Tauern Window, Eastern Alps). With increasing strain, seismic properties show a non-linear evolution resulting from interactions among structural, textural and mineralogical changes. Geometry and magnitude of seismic anisotropy are controlled by the interference of strongly anisotropic phyllosilicates with less anisotropic but more abundant quartz and plagioclase. The amphibolite-facies shear zone margin (> 500 • C) is free of major mineral transformations, and rather characterized by the development of S-C structure, inducing an orthorhombic symmetry to the V P distribution and up to 12 % AV P , with the fast axis parallel to lineation. AV S is maximized (6 %) in the two directions parallel to the C-planes. Further deformation under greenschist-facies (450-500 • C) induces significant crystallization of muscovite, chlorite, and albite. A dominant hexagonal symmetry develops, with a fast V P plane and a fast S-wave polarization plane along the C-planes. AV P and AV S exceed 10 %. The computed elastic tensor of the whole shear zone emphasizes the importance of the strain gradient on the seismic signature of the overall structure. In our model, the bulk seismic properties are dominated by the orthorhombic symmetry of the protomylonitic zone rather than by the ultramylonite core with hexagonal symmetry (C-plane), which may explain why seismic studies of shear zones commonly involve a fast velocity plane oblique to the layer boundaries.