Single-shot quantitative aberration and scattering length measurements in mouse brain tissues using an extended-source Shack-Hartmann wavefront sensor - Archive ouverte HAL Access content directly
Journal Articles Optics Express Year : 2022

Single-shot quantitative aberration and scattering length measurements in mouse brain tissues using an extended-source Shack-Hartmann wavefront sensor

Fabrice Harms
  • Function : Author
Antoine Hubert
  • Function : Author
Mathias Mercier
  • Function : Author
Laurent Bourdieu
Alexandra Fragola
  • Function : Author

Abstract

Deep fluorescence imaging in mammalian brain tissues remains challenging due to scattering and optical aberration-induced loss in signal and resolution. Correction of aberrations using adaptive optics (AO) requires their reliable measurement in the tissues. Here, we show that an extended-source Shack-Hartmann wavefront sensor (ESSH) allows quantitative aberration measurements through fixed brain slices with a thickness up to four times their scattering length. We demonstrate in particular that this wavefront measurement method based on image correlation is more robust to scattering compared to the standard centroid-based approach. Finally, we obtain a measurement of the tissue scattering length taking advantage of the geometry of a Shack-Hartmann sensor.
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Dates and versions

hal-03608265 , version 1 (14-03-2022)
hal-03608265 , version 2 (24-06-2022)

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Sophia Imperato, Fabrice Harms, Antoine Hubert, Mathias Mercier, Laurent Bourdieu, et al.. Single-shot quantitative aberration and scattering length measurements in mouse brain tissues using an extended-source Shack-Hartmann wavefront sensor. Optics Express, 2022, ⟨10.1364/OE.456651⟩. ⟨hal-03608265v2⟩
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