Single-shot quantitative aberration and scattering length measurements in mouse brain tissues using an extended-source Shack-Hartmann wavefront sensor - Archive ouverte HAL
Article Dans Une Revue Optics Express Année : 2022

Single-shot quantitative aberration and scattering length measurements in mouse brain tissues using an extended-source Shack-Hartmann wavefront sensor

Fabrice Harms
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Antoine Hubert
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Mathias Mercier
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Laurent Bourdieu
Alexandra Fragola
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Résumé

Deep fluorescence imaging in mammalian brain tissues remains challenging due to scattering and optical aberration-induced loss in signal and resolution. Correction of aberrations using adaptive optics (AO) requires their reliable measurement in the tissues. Here, we show that an extended-source Shack-Hartmann wavefront sensor (ESSH) allows quantitative aberration measurements through fixed brain slices with a thickness up to four times their scattering length. We demonstrate in particular that this wavefront measurement method based on image correlation is more robust to scattering compared to the standard centroid-based approach. Finally, we obtain a measurement of the tissue scattering length taking advantage of the geometry of a Shack-Hartmann sensor.
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Dates et versions

hal-03608265 , version 1 (14-03-2022)
hal-03608265 , version 2 (24-06-2022)

Identifiants

Citer

Sophia Imperato, Fabrice Harms, Antoine Hubert, Mathias Mercier, Laurent Bourdieu, et al.. Single-shot quantitative aberration and scattering length measurements in mouse brain tissues using an extended-source Shack-Hartmann wavefront sensor. Optics Express, 2022, ⟨10.1364/OE.456651⟩. ⟨hal-03608265v2⟩
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