Discrimination of α-Thrombin and γ-Thrombin Using Aptamer-Functionalized Nanopore Sensing - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Analytical Chemistry Année : 2021

Discrimination of α-Thrombin and γ-Thrombin Using Aptamer-Functionalized Nanopore Sensing

Résumé

Protein detection and identification at the single-molecule level are major challenges in many biotechnological fields. Solid-state nanopores have raised attention as label-free biosensors with high sensitivity. Here, we use solid-state nanopore sensing to discriminate two closely related proteins, α-thrombin and γ-thrombin. We show that aptamer functionalization improves protein discrimination thanks to a significant difference in the relative current blockade amplitude. To enhance discrimination, we postprocessed the signals using machine learning and training algorithms and we were able to reach an accuracy of 98.8% using seven features and ensemble methods.

Domaines

Biotechnologies
Fichier non déposé

Dates et versions

hal-03607965 , version 1 (14-03-2022)

Identifiants

Citer

Lucile Reynaud, Aurélie Bouchet-Spinelli, Jean-Marc Janot, Arnaud Buhot, Sebastien Balme, et al.. Discrimination of α-Thrombin and γ-Thrombin Using Aptamer-Functionalized Nanopore Sensing. Analytical Chemistry, 2021, 93 (22), pp.7889-7897. ⟨10.1021/acs.analchem.1c00461⟩. ⟨hal-03607965⟩
27 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More