Acoustical tweezers based on focused acoustical vortices open some tremendous perspectives for the in vitro and in vivo remote manipulation of millimetric down to micrometric objects, with com- bined selectivity and applied forces out of reach with any other contactless manipulation technique. Yet, the synthesis of ultra-high frequency acoustical vortices to manipulate precisely micrometric objects remains a major challenge. In this paper, the synthesis of a 250 MHz acoustical vortex is achieved with an active holographic source based on spiraling interdigitated transducers. It is shown that this ultra-high frequency vortex enables to trap and position individual particles in a standard microscopy environment with high spatial selectivity and nanoNewton forces. This work opens perspectives to explore acoustic force spectroscopy in some force ranges that were not accessible before.