Pre-B cell receptor acts as a selectivity switch for Galectin-1 at the pre-B cell surface - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2022

Pre-B cell receptor acts as a selectivity switch for Galectin-1 at the pre-B cell surface

Résumé

Abstract Galectins are glycan binding proteins translating the sugar-encoded information of cellular glycoconjugates into many physiological activities including immunity, cell migration, and signaling. During early B lymphocytes (BL) development at the pre-B cell stage, BL express the pre-B cell receptor (pre-BCR) and are supported by mesenchymal stromal cells secreting Galectin-1 (Gal-1). Gal-1 interacts with glycosylated receptors from stromal and pre-B cell surfaces but also with the pre-BCR through a direct carbohydrate-independent contact. How this interaction might interplay with the glycan-decoding function of Gal-1 is unknown. Here, we investigated Gal-1 binding to cell surface ligands using NMR spectroscopy on native membranes. We showed that pre-BCR regulates Gal-1 binding to specifically target α2,3-sialylated receptors on pre-B cells. Upon pre-BCR interaction, dynamic changes resulted in additional contacts with α2,3-sialylated glycans converting Gal-1 from an exo- to an endo-type lectin. Remarkably, this selectivity switch is able to promote pre-B cell survival. Altogether, we shed light on a new mechanism allowing fine-tuning of Galectin specificity at the cell surfaces.

Dates et versions

hal-03588373 , version 1 (24-02-2022)

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Pauline Touarin, Bastien Serrano, Audrey Courbois, Olivier Bornet, Qian Chen, et al.. Pre-B cell receptor acts as a selectivity switch for Galectin-1 at the pre-B cell surface. 2022. ⟨hal-03588373⟩
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