Cal-520FF is the Present Optimal Ca2+ Indicator for Ultrafast Ca2+ Imaging and Optical Measurement of Ca2+ Currents - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Fluorescence Année : 2021

Cal-520FF is the Present Optimal Ca2+ Indicator for Ultrafast Ca2+ Imaging and Optical Measurement of Ca2+ Currents

Résumé

Ultrafast Ca2+ imaging using low-affinity fluorescent indicators allows the precise measurement of the kinetics of fast Ca2+ currents mediated by voltage-gated Ca2+ channels. Thus far, only a few indicators provided fluorescence transients with sufficient signal-to-noise ratio necessary to achieve this measurement, with Oregon Green BAPTA-5N exhibiting the best performance. Here we evaluated the performance of the low-affinity Ca2+ indicator Cal-520FF to record fast Ca2+ signals and to measure the kinetics of Ca2+ currents. Compared to Oregon Green BAPTA-5N and to Fluo4FF, Cal-520FF offers a superior signal-to-noise-ratio providing the optimal characteristics for this important type of biophysical measurement. This ability is the result of a relatively high fluorescence at zero Ca2+, necessary to detect enough photons at short exposure windows, and a high dynamic range leading to large fluorescence transients associated with short Ca2+ influx periods. We conclude that Cal-520FF is at present the optimal commercial low-affinity Ca2+ indicator for ultrafast Ca2+ imaging applications.
Fichier principal
Vignette du fichier
Blömer_Filipis_Canepari_revised.pdf (302.55 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03578117 , version 1 (17-02-2022)

Identifiants

Citer

Laila Ananda Blömer, Luiza Filipis, Marco Canepari. Cal-520FF is the Present Optimal Ca2+ Indicator for Ultrafast Ca2+ Imaging and Optical Measurement of Ca2+ Currents. Journal of Fluorescence, 2021, 31 (3), pp.619-623. ⟨10.1007/s10895-021-02701-8⟩. ⟨hal-03578117⟩

Collections

UGA CNRS LIPHY ANR
16 Consultations
136 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More