Reproducibility of high-resolution laser speckle contrast imaging to assess cutaneous microcirculation for wound healing monitoring in mice - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Microvascular Research Année : 2022

Reproducibility of high-resolution laser speckle contrast imaging to assess cutaneous microcirculation for wound healing monitoring in mice

Résumé

Objective: Laser speckle contrast imaging (LSCI) combines an excellent spatial and temporal resolution, with excellent reproducibility in humans. Recently, high-resolution LSCI (LSCI-HR), coupled or not with oximetry, have been marketed. They are promising approaches to assess wound healing, especially in rodents. However, their reproducibility and performance against a reference technique remain unknown. Methods: Healthy skin perfusion was evaluated at day 0 and repeated at day 2, using LSCI-HR, high-resolution LSCI with oximetry by reflectance spectrometry (LSCI-OX), compared with laser Doppler imaging (LDI) as a reference. In a second experiment, cutaneous perfusion was measured daily during 8 days after wounding at two different sites. The reproducibility of haemoglobin oxygenation with LSCI-OX was also assessed in the two experiments. Reproducibility was expressed as within-subject coefficients of variation (CV, in %). Results: The inter-day reproducibility of healthy skin perfusion was better when assessed with LSCI-HR and LSCIOX, compared to LDI (CVs between 12 and 17% and between 26 and 29%, respectively). Inter-site reproducibility of perfusion during wound healing was also better with LSCI-HR compared to LDI (CV = 12% and 23%, respectively). Finally, we observed a good, positive correlation between perfusion measured with LDI and LSCIHR on the periulcer area (average r = 0.77 ± 0.24). Conclusion: Recently developed high-resolution LSCI devices provide good reproducibility to assess healthy and wounded skin perfusion in mice. However, the reproducibility of haemoglobin oxygenation is poor.
Fichier non déposé

Dates et versions

hal-03561927 , version 1 (08-02-2022)

Identifiants

Citer

Axel Couturier, Raphaël Bouvet, Jean-Luc Cracowski, Matthieu Roustit. Reproducibility of high-resolution laser speckle contrast imaging to assess cutaneous microcirculation for wound healing monitoring in mice. Microvascular Research, 2022, 141, pp.104319. ⟨10.1016/j.mvr.2022.104319⟩. ⟨hal-03561927⟩

Collections

UGA ANR
17 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More