Novel Optical Biosensor Based on a Nano-Gold Coated by Schiff Base Doped in Sol/Gel Matrix for Sensitive Screening of Oncomarker CA-125 - Archive ouverte HAL
Article Dans Une Revue ACS Omega Année : 2021

Novel Optical Biosensor Based on a Nano-Gold Coated by Schiff Base Doped in Sol/Gel Matrix for Sensitive Screening of Oncomarker CA-125

M.N. Abou-Omar
  • Fonction : Auteur
M.S. Attia
  • Fonction : Auteur
H.G. Afify
  • Fonction : Auteur
M.A. Amin
  • Fonction : Auteur
E.H. Mohamed
  • Fonction : Auteur

Résumé

The urge for sensitive, facile, minimally invasive, and fast detection method of CA-125, a significant and crucial biomarker in ovarian malignancy, is currently substantial. This paper describes the detailed construction and characterization of a newly designed optical nano-biosensor to detect CA-125 accurately and sensitively. The fabricated sensor consists of a nano-gold thin film doped into a matrix of sol-gel, exhibiting a centered fluorescence band at 423 nm when excited at 340 nm. The quantification of CA-125 relies on its quenching ability of this fluorescence signal. The sensor was challenged to evaluate its sensitivity and specificity in detecting CA-125 present in samples collected from ovarian cancer diagnosed patients and compared to samples from healthy women as a control. Our findings revealed that the developed biosensor had a sensitivity of 97.35% and a specificity of 94.29%. Additionally, a wide linearity range over 2.0-127.0 U mL-1 for CA-125 was achieved with a detection limit of 1.45 U mL-1. Furthermore, the sensor could successfully discriminate samples between healthy and diseased people, which demonstrates its suitability in CA-125 assessment. © 2021 The Authors. Published by American Chemical Society.
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hal-03546308 , version 1 (02-05-2024)

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M.N. Abou-Omar, M.S. Attia, H.G. Afify, M.A. Amin, Rabah Boukherroub, et al.. Novel Optical Biosensor Based on a Nano-Gold Coated by Schiff Base Doped in Sol/Gel Matrix for Sensitive Screening of Oncomarker CA-125. ACS Omega, 2021, 6 (32), pp.20812-20821. ⟨10.1021/acsomega.1c01974⟩. ⟨hal-03546308⟩
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