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Article Dans Une Revue Analytical Chemistry Année : 2017

Taylor Dispersion Analysis of Polysaccharides Using Backscattering Interferometry

Résumé

Taylor dispersion analysis (TDA) allows the determination of the molecular diffusion coefficient (D) or the hydrodynamic radius (Rh) of a solute from the peak broadening of a plug of solute in a laminar Poiseuille flow. The main limitation plaguing the broader applicability of TDA is the lack of a sensitive detection modality. UV absorption is typically used with TDA but is only suitable for UV-absorbing or derivatized compounds. In this work, we present a development of the TDA method for non-UV absorbing compounds by using a universal detector based on refractive index (RI) sensing with backscattering interferometry (BSI). BSI was interfaced to a capillary electrophoresis-UV instrument using a polyimide coated fused silica capillary and an in-house designed flow-cell assembly. Polysaccharides were selected to demonstrate the application of TDA-BSI for size characterization. Under the conditions of validity of TDA, D and Rh average values and the entire Rh distributions were obtained from the (poly)saccharide taylorgrams, including non-UV absorbing polymers.

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Chimie
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Dates et versions

hal-03537943 , version 1 (20-01-2022)

Identifiants

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Phoonthawee Saetear, Joseph Chamieh, Michael Kammer, Thomas Manuel, Jean-Philippe Biron, et al.. Taylor Dispersion Analysis of Polysaccharides Using Backscattering Interferometry. Analytical Chemistry, 2017, 89 (12), pp.6710-6718. ⟨10.1021/acs.analchem.7b00946⟩. ⟨hal-03537943⟩
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