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Journal Articles Scientific Reports Year : 2015

Live cell immunogold labelling of RNA polymerase II

Abstract

Labeling nuclear proteins with electron dense probes in living cells has been a major challenge due to their inability to penetrate into nuclei. We developed a lipid-based approach for delivering antibodies coupled to 0.8 nm ultrasmall gold particles into the nucleus to label RNA polymerase II. Focussed Ion Beam slicing coupled to Scanning Electron Microscopy (FIB/SEM) enabled visualization of entire cells with probe localization accuracy in the 10 nm range.
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hal-03535681 , version 1 (19-01-2022)

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Igor Orlov, Andreas Schertel, Guy Zuber, Bruno Klaholz, Robert Drillien, et al.. Live cell immunogold labelling of RNA polymerase II. Scientific Reports, 2015, 5 (1), pp.8324. ⟨10.1038/srep08324⟩. ⟨hal-03535681⟩
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