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Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2022

Dengue virus infection modifies mosquito blood-feeding behavior to increase transmission to the host

Résumé

Mosquito blood-feeding behavior is a key determinant of the epidemiology of dengue viruses (DENV), the most-prevalent mosquito-borne viruses. However, despite its importance, how DENV infection influences mosquito blood-feeding and, consequently, transmission remains unclear. Here, we developed a high-resolution, video-based assay to observe the blood-feeding behavior of Aedes aegypti mosquitoes on mice. We then applied multivariate analysis on the high-throughput, unbiased data generated from the assay to ordinate behavioral parameters into complex behaviors. We showed that DENV infection increases mosquito attraction to the host and hinders its biting efficiency, the latter resulting in the infected mosquitoes biting more to reach similar blood repletion as uninfected mosquitoes. To examine how increased biting influences DENV transmission to the host, we established an in vivo transmission model with immuno-competent mice and demonstrated that successive short probes result in multiple transmissions. Finally, to determine how DENV-induced alterations of host-seeking and biting behaviors influence dengue epidemiology, we integrated the behavioral data within a mathematical model. We calculated that the number of infected hosts per infected mosquito, as determined by the reproduction rate, tripled when mosquito behavior was influenced by DENV infection. Taken together, this multidisciplinary study details how DENV infection modulates mosquito blood-feeding behavior to increase vector capacity, proportionally aggravating DENV epidemiology. By elucidating the contribution of mosquito behavioral alterations on DENV transmission to the host, these results will inform epidemiological modeling to tailor improved interventions against dengue.
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Origine : Publication financée par une institution
Licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

Dates et versions

hal-03521021 , version 1 (11-01-2022)
hal-03521021 , version 2 (25-10-2022)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

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Benjamin Wong Wei Xiang, Wilfried Saron, James Stewart, Arthur Hain, Varsha Walvekar, et al.. Dengue virus infection modifies mosquito blood-feeding behavior to increase transmission to the host. Proceedings of the National Academy of Sciences of the United States of America, 2022, 119 (3), pp.e2117589119. ⟨10.1073/pnas.2117589119⟩. ⟨hal-03521021v2⟩
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