To improve membrane fouling management, the NaClO-assisted backwash has been developed to improve permeability maintenance and reduce the need for intensive chemical cleanings. This study is aimed to focus on the efficiency of NaClO-assisted backwash in real UF pilot scale and with periodic classic backwash (CB) and air backwash (AB). The impacts on hydraulic filtration performance, physicochemical properties of membrane material under different addition frequencies of NaClO, and the performance of chlorinated CB and AB will be discussed. In result, 10 mg Cl2 L−1 NaClO addition in backwash water is confirmed to greatly improve the overall filtration performance and backwash cleaning efficiency. One condition stands out from the other due to better control of irreversible fouling, less NaClO consumption in 10 years prediction, sustainable and adaptable filtration performance, and less potential damage on the physicochemical properties of the membrane. Additionally, it can be inferred from this experiment that frequent contact with NaClO induced some degradation on the PES-made UF membrane surface properties. To retain the best state of UF membrane on anti-fouling and qualified production, the optimized condition with more frequent NaClO contact was not suggested for long-term filtration.