A reliable amplification technique for the characterization of genomic DNA sequences flanking insertion sequences - Archive ouverte HAL
Article Dans Une Revue FEMS Microbiology Letters Année : 1998

A reliable amplification technique for the characterization of genomic DNA sequences flanking insertion sequences

Résumé

A simple and efficient ligation-mediated PCR (LMPCR) is described for amplifying DNA adjacent to known sequences. The method uses one primer specific for the known sequence and a second specific for a synthetic linker ligated to restricted genomic DNA. Perkin-Elmer AmpliTaq Gold polymerase is used to minimize non-specific primer annealing and amplification. This LMPCR method was successfully applied to isolate DNA sequences flanking mobile elements present in mycobacterial mutants generated by transposon mutagenesis.

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Bactériologie
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Dates et versions

hal-03513952 , version 1 (06-01-2022)

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Guy Prod'Hom, Béatrice Lagier, V. Pelicic, Allan J. Hance, Brigitte Gicquel, et al.. A reliable amplification technique for the characterization of genomic DNA sequences flanking insertion sequences. FEMS Microbiology Letters, 1998, 158 (1), pp.75-81. ⟨10.1111/j.1574-6968.1998.tb12803.x⟩. ⟨hal-03513952⟩
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