Plasmodium infection is associated with cross‑reactive antibodies to carbohydrate epitopes on the SARS‑CoV‑2 Spike protein
Sarah Lapidus
(1)
,
Feimei Liu
(2)
,
Arnau Casanovas-Massana
(1)
,
Yile Dai
(2)
,
John D. Huck
(2)
,
Carolina Lucas
(2)
,
Jon Klein
(2)
,
Renata B. Filler
(2)
,
Madison S. Strine
(2)
,
Mouhamad Sy
(3)
,
Awa B. Deme
(3)
,
Aida S. Badiane
(3)
,
Baba Dieye
(3)
,
Ibrahima Mbaye Ndiaye
(3)
,
Younous Diedhiou
(3)
,
Amadou Moctar Mbaye
(3)
,
Cheikh Tidiane Diagne
(4)
,
Inès Vigan‑womas
(5)
,
Alassane Mbengue
(5)
,
Bacary D. Sadio
(6)
,
Moussa M. Diagne
(6)
,
Adam J. Moore
(1)
,
Khadidiatou Mangou
(5)
,
Fatoumata Diallo
(5)
,
Seynabou D. Sene
(5)
,
Mariama N. Pouye
(5)
,
Rokhaya Faye
(5)
,
Babacar Diouf
(5)
,
Nivison Jr Nery
(7, 8)
,
Federico Costa
(1, 7, 8)
,
Mitermayer Reis
(1, 7, 8)
,
M. Catherine Muenker
(1)
,
Daniel Z. Hodson
(1)
,
Yannick Mbarga
(9)
,
Ben Z. Katz
(10, 11)
,
Jason R. Andrews
(12)
,
Melissa Campbell
(2)
,
Ariktha Srivathsan
(1)
,
Kathy Kamath
(13)
,
Elisabeth Baum-Jones
(13)
,
Ousmane Faye
(6)
,
Amadou Alpha Sall
(6)
,
Juan Carlos Quintero Vélez
(14)
,
Michael Cappello
(2)
,
Michael Wilson
(15)
,
Choukri Ben-Mamoun
(2)
,
Fabrice A. Somé
(16)
,
Roch K. Dabiré
(16)
,
Carole Else Eboumbou Moukoko
(17, 18)
,
Jean Bosco Ouédraogo
(16)
,
Yap Boum
(19, 20)
,
John Shon
(13)
,
Daouda Ndiaye
(3)
,
Adam Wisnewski
(2)
,
Sunil Parikh
(1)
,
Akiko Iwasaki
(2)
,
Craig B. Wilen
(2)
,
Albert I. Ko
(1, 8)
,
Aaron M. Ring
(2)
,
Amy K. Bei
(1, 3, 5)
1
YSPH -
Yale School of Public Health
2 YSM - Yale School of Medicine [New Haven, Connecticut]
3 UCAD - Université Cheikh Anta Diop de Dakar [Sénégal]
4 Diatropix [Dakar, Sénégal]
5 Pôle Immunophysiopathologie et Maladies Infectieuses
6 Département de virologie - Virology Department [Dakar]
7 UFBA - Universidade Federal da Bahia = Federal University of Bahia
8 IGM - Instituto Gonçalo Moniz / Gonçalo Moniz Research Centre - Fiocruz Bahia [Salvador, Brésil]
9 Douala Military Hospital
10 [Northwestern University Medical School] - Feinberg School of Medicine [Northwestern University, Evanston]
11 Ann & Robert H. Lurie Children's Hospital of Chicago
12 Stanford University
13 Serimmune [Goleta, CA]
14 Universidad de Antioquia = University of Antioquia [Medellín, Colombia]
15 University of Ghana
16 Institut de Recherche en Sciences de la Santé (IRSS) / Centre Muraz
17 Université de Douala
18 Centre Pasteur du Cameroun
19 University of Yaoundé [Cameroun]
20 Epicentre [Paris] [Médecins Sans Frontières]
2 YSM - Yale School of Medicine [New Haven, Connecticut]
3 UCAD - Université Cheikh Anta Diop de Dakar [Sénégal]
4 Diatropix [Dakar, Sénégal]
5 Pôle Immunophysiopathologie et Maladies Infectieuses
6 Département de virologie - Virology Department [Dakar]
7 UFBA - Universidade Federal da Bahia = Federal University of Bahia
8 IGM - Instituto Gonçalo Moniz / Gonçalo Moniz Research Centre - Fiocruz Bahia [Salvador, Brésil]
9 Douala Military Hospital
10 [Northwestern University Medical School] - Feinberg School of Medicine [Northwestern University, Evanston]
11 Ann & Robert H. Lurie Children's Hospital of Chicago
12 Stanford University
13 Serimmune [Goleta, CA]
14 Universidad de Antioquia = University of Antioquia [Medellín, Colombia]
15 University of Ghana
16 Institut de Recherche en Sciences de la Santé (IRSS) / Centre Muraz
17 Université de Douala
18 Centre Pasteur du Cameroun
19 University of Yaoundé [Cameroun]
20 Epicentre [Paris] [Médecins Sans Frontières]
Feimei Liu
- Fonction : Auteur
- PersonId : 819163
- ORCID : 0000-0003-2663-6544
Arnau Casanovas-Massana
- Fonction : Auteur
- PersonId : 806365
- ORCID : 0000-0002-3301-6143
John D. Huck
- Fonction : Auteur
- PersonId : 819839
- ORCID : 0000-0002-0755-3850
Awa B. Deme
- Fonction : Auteur
- PersonId : 819840
- ORCID : 0000-0002-3119-6419
Aida S. Badiane
- Fonction : Auteur
- PersonId : 799571
- ORCID : 0000-0002-1118-4998
Cheikh Tidiane Diagne
- Fonction : Auteur
- PersonId : 794492
- ORCID : 0000-0003-2119-9349
- IdRef : 177276819
Inès Vigan‑womas
- Fonction : Auteur
- PersonId : 736886
- IdHAL : ines-vigan-womas
- ORCID : 0000-0001-7848-9297
- IdRef : 241942020
Alassane Mbengue
- Fonction : Auteur
- PersonId : 799357
- ORCID : 0000-0002-4210-6167
- IdRef : 164805206
Jason R. Andrews
- Fonction : Auteur
- PersonId : 819841
- ORCID : 0000-0002-5967-251X
Akiko Iwasaki
- Fonction : Auteur
- PersonId : 766959
- ORCID : 0000-0002-7824-9856
Craig B. Wilen
- Fonction : Auteur
- PersonId : 819843
- ORCID : 0000-0003-2495-9403
Albert I. Ko
- Fonction : Auteur
- PersonId : 802512
- ORCID : 0000-0001-9023-2339
Aaron M. Ring
- Fonction : Auteur
- PersonId : 819844
- ORCID : 0000-0003-3699-2446
Amy K. Bei
Connectez-vous pour contacter l'auteur
- Fonction : Auteur correspondant
- PersonId : 1140243
Connectez-vous pour contacter l'auteur
Résumé
Sero-surveillance can monitor and project disease burden and risk. However, SARS-CoV-2 antibody test results can produce false positive results, limiting their efficacy as a sero-surveillance tool. False positive SARS-CoV-2 antibody results are associated with malaria exposure, and understanding this association is essential to interpret sero-surveillance results from malaria-endemic countries. Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity. Individuals with acute malaria infection generated substantial SARS-CoV-2 reactivity. Cross-reactivity was not associated with reactivity to other human coronaviruses or other SARS-CoV-2 proteins, as measured by peptide and protein arrays. ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein. The functional activity of cross-reactive antibodies measured by neutralization assays showed that cross-reactive antibodies did not neutralize SARS-CoV-2 in vitro. Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity. Overestimating population-level exposure to SARS-CoV-2 could lead to underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa.
| Origine | Publication financée par une institution |
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