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Article Dans Une Revue Ecological Indicators Année : 2018

Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea

Francesca Sbrana
  • Fonction : Auteur
Silvia Casabianca
  • Fonction : Auteur
Nikolla Gjeci
  • Fonction : Auteur
Samuela Capellacci
  • Fonction : Auteur
Ennio Ottaviani
  • Fonction : Auteur
Valentina Giussani
  • Fonction : Auteur
Laura Pugliese
  • Fonction : Auteur
Marie Abboud-Abi Saab
  • Fonction : Auteur
Rosella Bertolotto
  • Fonction : Auteur
Cecilia Totti
  • Fonction : Auteur
Stefano Accoroni
  • Fonction : Auteur
Magda Vila
  • Fonction : Auteur

Résumé

This paper describes the adoption and validation of two innovative methods for the automated count of Ostreopsis spp. concentration in sea water: a molecular assay based on RT-qPCR and an opto-electronic device implementing automatic recognition algorithms. The proposed approaches were tested on samples coming from different locations along the Mediterranean Sea and compared with the standard counting method based on microscopy observation by a taxonomy expert. The results demonstrate the effectiveness of both automatic approaches which provide a valuable tool, mostly cost and time effective, for the establishment of wide pan Mediterranean monitoring strategies of Ostreopsis spp. blooms. Moreover, the two automatic methods demonstrated the ability to discriminate for the presence of a different but similar species, O. fattorussoi, for which new species-specific qPCR primers were developed.

Domaines

Océanographie

Dates et versions

hal-03505068 , version 1 (30-12-2021)

Identifiants

Citer

Massimo Vassalli, Antonella Penna, Francesca Sbrana, Silvia Casabianca, Nikolla Gjeci, et al.. Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea. Ecological Indicators, 2018, 85, pp.1092-1100. ⟨10.1016/j.ecolind.2017.07.063⟩. ⟨hal-03505068⟩
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