Intercalibration of counting methods for Ostreopsis spp. blooms in the Mediterranean Sea
Massimo Vassalli
(1)
,
Antonella Penna
(2)
,
Francesca Sbrana
,
Silvia Casabianca
,
Nikolla Gjeci
,
Samuela Capellacci
,
Valentina Asnaghi
(3)
,
Ennio Ottaviani
(4)
,
Valentina Giussani
,
Laura Pugliese
,
Cecile Jauzein
(5)
,
Rodolphe Lemée
(5)
,
Mohamed Amine Hachani
(6, 7)
,
Souad Turki
(8)
,
Laury Acaf
(5)
,
Marie Abboud-Abi Saab
,
Anna Fricke
(5, 9)
,
Luisa Mangialajo
(9, 5)
,
Rosella Bertolotto
,
Cecilia Totti
,
Stefano Accoroni
,
Elisa Berdalet
(10)
,
Magda Vila
,
Mariachiara Chiantore
(3)
1
James Watt School of Engineering [Univ Glasgow]
2 Università di Urbino
3 DISTAV - Department of Earth, Environmental and Life Sciences
4 OnAIR srl
5 LOV - Laboratoire d'océanographie de Villefranche
6 Institut National des Sciences et Technologies de la Mer
7 LCE - Laboratoire Chrono-environnement (UMR 6249)
8 OGS - Istituto Nazionale di Oceanografia e di Geofisica Sperimentale
9 ECOMERS - Ecosystèmes Côtiers Marins et Réponses aux Stress
10 CSIC - Consejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain]
2 Università di Urbino
3 DISTAV - Department of Earth, Environmental and Life Sciences
4 OnAIR srl
5 LOV - Laboratoire d'océanographie de Villefranche
6 Institut National des Sciences et Technologies de la Mer
7 LCE - Laboratoire Chrono-environnement (UMR 6249)
8 OGS - Istituto Nazionale di Oceanografia e di Geofisica Sperimentale
9 ECOMERS - Ecosystèmes Côtiers Marins et Réponses aux Stress
10 CSIC - Consejo Superior de Investigaciones Cientificas [España] = Spanish National Research Council [Spain]
Massimo Vassalli
- Fonction : Auteur
- PersonId : 762608
- ORCID : 0000-0002-3063-4376
Francesca Sbrana
- Fonction : Auteur
Silvia Casabianca
- Fonction : Auteur
Nikolla Gjeci
- Fonction : Auteur
Samuela Capellacci
- Fonction : Auteur
Valentina Giussani
- Fonction : Auteur
Laura Pugliese
- Fonction : Auteur
Rodolphe Lemée
- Fonction : Auteur
- PersonId : 176762
- IdHAL : rodolphe-lemee
- ORCID : 0000-0002-7800-2220
- IdRef : 169814637
Marie Abboud-Abi Saab
- Fonction : Auteur
Rosella Bertolotto
- Fonction : Auteur
Cecilia Totti
- Fonction : Auteur
Stefano Accoroni
- Fonction : Auteur
Elisa Berdalet
- Fonction : Auteur
- PersonId : 791189
- ORCID : 0000-0003-1123-9706
- IdRef : 221081992
Magda Vila
- Fonction : Auteur
Résumé
This paper describes the adoption and validation of two innovative methods for the automated count of Ostreopsis spp. concentration in sea water: a molecular assay based on RT-qPCR and an opto-electronic device implementing automatic recognition algorithms. The proposed approaches were tested on samples coming from different locations along the Mediterranean Sea and compared with the standard counting method based on microscopy observation by a taxonomy expert. The results demonstrate the effectiveness of both automatic approaches which provide a valuable tool, mostly cost and time effective, for the establishment of wide pan Mediterranean monitoring strategies of Ostreopsis spp. blooms. Moreover, the two automatic methods demonstrated the ability to discriminate for the presence of a different but similar species, O. fattorussoi, for which new species-specific qPCR primers were developed.