Time-lapse and cell ablation reveal the role of cell interactions in fly glia migration and proliferation - Archive ouverte HAL Access content directly
Journal Articles Development (Cambridge, England) Year : 2004

Time-lapse and cell ablation reveal the role of cell interactions in fly glia migration and proliferation

Abstract

Migration and proliferation have been mostly explored in culture systems or fixed preparations. We present a simple genetic model, the chains of glia moving along fly wing nerves, to follow such dynamic processes by time-lapse in the whole animal. We show that glia undergo extensive cytoskeleton and mitotic apparatus rearrangements during division and migration. Single cell labelling identifies different glia: pioneers with high filopodial,exploratory, activity and, less active followers. In combination with time-lapse, altering this cellular environment by genetic means or cell ablation has allowed to us define the role of specific cell-cell interactions. First, neurone-glia interactions are not necessary for glia motility but do affect the direction of migration. Second, repulsive interactions between glia control the extent of movement. Finally, autonomous cues control proliferation.
Fichier principal
Vignette du fichier
5127.pdf (2.3 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03447948 , version 1 (15-12-2021)

Identifiers

Cite

Benoît Aigouy, Véronique van de Bor, Marcel Boeglin, Angela Giangrande. Time-lapse and cell ablation reveal the role of cell interactions in fly glia migration and proliferation. Development (Cambridge, England), 2004, 131 (20), pp.5127-5138. ⟨10.1242/dev.01398⟩. ⟨hal-03447948⟩
11 View
8 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More