Abstract Consideration of copper storage and availability in vineyard soils to assess its ecotoxicity. Comments on the article by Karimi, et al (2021)-Ecotoxicity of copper input and accumulation for soil biodiversity in vineyards. Etude et Gestion des Sols, 28 (1), p. 71-92. Studies of the ecotoxicological impact of copper in vineyard soils are generally based on the effect of adding a specific dose of copper (Cu). However, the soil properties and the dynamics of Cu storage in the soil must also be taken into account to determine at the vineyard and wine-growing region level the ecotoxicity thresholds that should not be exceeded to maintain good soil biological quality. The use of Cu up to 28 kg/ha for seven years, in accordance with current European regulations, generally postpones the deadline for exceeding the Cu ecotoxicity threshold impacting soil biodiversity. However, an improvement in the quality and sustainability of wine production in Europe requires an adapted local use and a more specific evaluation of the impact of copper accounting for the trajectory of Cu accumulation and availability in the soil.