Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study - Archive ouverte HAL
Article Dans Une Revue European Biophysics Journal Année : 2021

Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study

Blanca López-Méndez (1) , Bruno Baron (2) , Chad Brautigam (3) , Thomas Jowitt (4) , Stefan Knauer (5) , Stephan Uebel (6) , Mark Williams (7) , Arthur Sedivy (8) , Olga Abian (9) , Celeste Abreu (10) , Malgorzata Adamczyk (11) , Wojciech Bal (12) , Sylvie Berger (13) , Alexander Buell (14) , Carlo Carolis (15) , Tina Daviter (7, 16) , Alexander Fish (17) , Maria Garcia-Alai (18) , Christian Guenther (18) , Josef Hamacek (19) , Jitka Holková (20) , Josef Houser (20) , Chris Johnson (21) , Sharon Kelly (22) , Andrew Leech (23) , Caroline Mas (24) , Daumantas Matulis (25) , Stephen Mclaughlin (21) , Roland Montserret (26) , Rouba Nasreddine (27) , Reine Nehmé (27) , Quyen Nguyen (13) , David Ortega-Alarcón (9) , Kathryn Perez (28) , Katja Pirc (29) , Grzegorz Piszczek (30) , Marjetka Podobnik (29) , Natalia Rodrigo (15) , Jasmina Rokov-Plavec (31) , Susanne Schaefer (5) , Tim Sharpe (32) , June Southall (22) , David Staunton (33) , Pedro Tavares (34) , Ondrej Vanek (10) , Michael Weyand (5) , Di Wu (30)
1 UCPH - University of Copenhagen = Københavns Universitet
2 Biophysique Moléculaire (plateforme) - Molecular Biophysics (platform)
3 University of Texas Southwestern Medical Center [Dallas]
4 University of Manchester [Manchester]
5 Universität Bayreuth [Deutschland] = University of Bayreuth [Germany] = Université de Bayreuth [Allemagne]
6 MPIB - Max-Planck-Institut für Biochemie = Max Planck Institute of Biochemistry
7 University of London [London]
8 Vienna Biocenter Core Facilities
9 Universidad de Zaragoza = University of Zaragoza [Saragossa University] = Université de Saragosse
10 UK - Univerzita Karlova [Praha, Česká republika] = Charles University [Prague, Czech Republic]
11 Warsaw University of Technology [Warsaw]
12 Institute of Biochemistry and Biophysics
13 IRS - Institut de Recherches SERVIER
14 DTU - Danmarks Tekniske Universitet = Technical University of Denmark
15 CRG - Centre for Genomic Regulation - Centre de Regulació Genòmica [Barcelona]
16 The institute of cancer research [London]
17 NKI - Netherlands Cancer Institute
18 EMBL Hamburg
19 CBM - Centre de biophysique moléculaire
20 CEITEC MU - Central European Institute of Technology [Brno]
21 LMB - MRC Laboratory of Molecular Biology [Cambridge, UK]
22 University of Glasgow
23 University of York [York, UK]
24 ISBG - Integrated Structural Biology Grenoble
25 Vilnius University [Vilnius]
26 IBCP - Institut de biologie et chimie des protéines [Lyon]
27 ICOA - Institut de Chimie Organique et Analytique
28 EMBL Heidelberg
29 KI - National Institute of Chemistry [Llubljana]
30 NHLBI - National Heart, Lung, and Blood Institute [Bethesda]
31 University of Zagreb
32 Unibas - Université de Bâle = University of Basel = Basel Universität
33 University of Oxford
34 FCT - Faculdade de Ciências e Tecnologia [Faro]
Blanca López-Méndez
University of Copenhagen = Københavns Universitet
Bruno Baron
  • Fonction : Auteur
  • PersonId : 757146
  • IdRef : 163546797
Biophysique Moléculaire (plateforme) - Molecular Biophysics (platform)
Chad Brautigam
University of Texas Southwestern Medical Center [Dallas]
Thomas Jowitt
University of Manchester [Manchester]
Stefan Knauer
Universität Bayreuth [Deutschland] = University of Bayreuth [Germany] = Université de Bayreuth [Allemagne]
Stephan Uebel
Max-Planck-Institut für Biochemie = Max Planck Institute of Biochemistry
Mark Williams
University of London [London]
Arthur Sedivy
  • Fonction : Auteur correspondant
  • PersonId : 1139920

Connectez-vous pour contacter l'auteur
Vienna Biocenter Core Facilities
Olga Abian
Universidad de Zaragoza = University of Zaragoza [Saragossa University] = Université de Saragosse
Celeste Abreu
Univerzita Karlova [Praha, Česká republika] = Charles University [Prague, Czech Republic]
Malgorzata Adamczyk
Warsaw University of Technology [Warsaw]
Wojciech Bal
Institute of Biochemistry and Biophysics
Sylvie Berger
  • Fonction : Auteur
Institut de Recherches SERVIER
Alexander Buell
Danmarks Tekniske Universitet = Technical University of Denmark
Carlo Carolis
  • Fonction : Auteur
Centre for Genomic Regulation - Centre de Regulació Genòmica [Barcelona]
Tina Daviter
  • Fonction : Auteur
University of London [London]
The institute of cancer research [London]
Alexander Fish
Netherlands Cancer Institute
Maria Garcia-Alai
EMBL Hamburg
Christian Guenther
  • Fonction : Auteur
EMBL Hamburg
Josef Hamacek
Centre de biophysique moléculaire
CV
Jitka Holková
  • Fonction : Auteur
Central European Institute of Technology [Brno]
Josef Houser
Central European Institute of Technology [Brno]
Chris Johnson
  • Fonction : Auteur
MRC Laboratory of Molecular Biology [Cambridge, UK]
Sharon Kelly
  • Fonction : Auteur
University of Glasgow
Andrew Leech
University of York [York, UK]
Caroline Mas
Integrated Structural Biology Grenoble
Daumantas Matulis
Vilnius University [Vilnius]
Stephen Mclaughlin
  • Fonction : Auteur
MRC Laboratory of Molecular Biology [Cambridge, UK]
Roland Montserret
Institut de biologie et chimie des protéines [Lyon]
Rouba Nasreddine
Institut de Chimie Organique et Analytique
Reine Nehmé
Institut de Chimie Organique et Analytique
CV
Quyen Nguyen
  • Fonction : Auteur
Institut de Recherches SERVIER
David Ortega-Alarcón
  • Fonction : Auteur
Universidad de Zaragoza = University of Zaragoza [Saragossa University] = Université de Saragosse
Kathryn Perez
  • Fonction : Auteur
EMBL Heidelberg
Katja Pirc
National Institute of Chemistry [Llubljana]
Grzegorz Piszczek
National Heart, Lung, and Blood Institute [Bethesda]
Marjetka Podobnik
National Institute of Chemistry [Llubljana]
Natalia Rodrigo
  • Fonction : Auteur
Centre for Genomic Regulation - Centre de Regulació Genòmica [Barcelona]
Jasmina Rokov-Plavec
University of Zagreb
Susanne Schaefer
  • Fonction : Auteur
Universität Bayreuth [Deutschland] = University of Bayreuth [Germany] = Université de Bayreuth [Allemagne]
Tim Sharpe
  • Fonction : Auteur
Université de Bâle = University of Basel = Basel Universität
June Southall
  • Fonction : Auteur
University of Glasgow
David Staunton
  • Fonction : Auteur
University of Oxford
Pedro Tavares
  • Fonction : Auteur
Faculdade de Ciências e Tecnologia [Faro]
Ondrej Vanek
Univerzita Karlova [Praha, Česká republika] = Charles University [Prague, Czech Republic]
Michael Weyand
  • Fonction : Auteur
Universität Bayreuth [Deutschland] = University of Bayreuth [Germany] = Université de Bayreuth [Allemagne]
Di Wu
  • Fonction : Auteur
National Heart, Lung, and Blood Institute [Bethesda]

Résumé

Microscale thermophoresis (MST), and the closely related Temperature Related Intensity Change (TRIC), are synonyms for a recently developed measurement technique in the field of biophysics to quantify biomolecular interactions, using the (capillary-based) NanoTemper Monolith and (multiwell plate-based) Dianthus instruments. Although this technique has been extensively used within the scientific community due to its low sample consumption, ease of use, and ubiquitous applicability, MST/TRIC has not enjoyed the unambiguous acceptance from biophysicists afforded to other biophysical techniques like isothermal titration calorimetry (ITC) or surface plasmon resonance (SPR). This might be attributed to several facts, e.g., that various (not fully understood) effects are contributing to the signal, that the technique is licensed to only a single instrument developer, NanoTemper Technology, and that its reliability and reproducibility have never been tested independently and systematically. Thus, a working group of ARBRE-MOBIEU has set up a benchmark study on MST/TRIC to assess this technique as a method to characterize biomolecular interactions. Here we present the results of this study involving 32 scientific groups within Europe and two groups from the US, carrying out experiments on 40 Monolith instruments , employing a standard operation procedure and centrally prepared samples. A protein–small molecule interaction, a newly developed protein–protein interaction system and a pure dye were used as test systems. We characterized the instrument properties and evaluated instrument performance, reproducibility, the effect of different analysis tools, the influence of the experimenter during data analysis, and thus the overall reliability of this method.

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Biophysique Instrumentations et Détecteurs [physics.ins-det]
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https://hal.science/hal-03481822

Soumis le : vendredi 17 décembre 2021-11:18:54

Dernière modification le : lundi 9 décembre 2024-03:39:06

Archivage à long terme le : vendredi 18 mars 2022-18:48:00

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hal-03481822 , version 1 (17-12-2021)

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Blanca López-Méndez, Bruno Baron, Chad Brautigam, Thomas Jowitt, Stefan Knauer, et al.. Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study. European Biophysics Journal, 2021, 50 (3-4), pp.411-427. ⟨10.1007/s00249-021-01532-6⟩. ⟨hal-03481822⟩

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