Effect of soluble adenylyl cyclase (ADCY10) inhibitors on the LH-stimulated cAMP synthesis in Mltc-1 leydig cell line - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue International Journal of Molecular Sciences Année : 2021

Effect of soluble adenylyl cyclase (ADCY10) inhibitors on the LH-stimulated cAMP synthesis in Mltc-1 leydig cell line

Résumé

In contrast to all transmembrane adenylyl cyclases except ADCY9, the cytosolic soluble adenylyl cyclase (ADCY10) is insensitive to forskolin stimulation and is uniquely modulated by calcium and bicarbonate ions. In the present paper, we focus on ADCY10 localization and a kinetic analysis of intracellular cAMP accumulation in response to human LH in the absence or presence of four different ADCY10 inhibitors (KH7, LRE1, 2-CE and 4-CE) in MTLC-1 cells. ADCY10 was immuno-detected in the cytoplasm of MLTC-1 cells and all four inhibitors were found to inhibit LH-stimulated cAMP accumulation and progesterone level in MLTC-1 and testosterone level primary Leydig cells. Interestingly, similar inhibitions were also evidenced in mouse testicular Leydig cells. In contrast, the tmAC-specific inhibitors ddAdo3′ and ddAdo5′, even at high concentration, exerted weak or no inhibition on cAMP accumulation, suggesting an important role of ADCY10 relative to tmACs in the MLTC-1 response to LH. The strong synergistic effect of HCO3− under LH stimulation further supports the involvement of ADCY10 in the response to LH.

Dates et versions

hal-03468377 , version 1 (07-12-2021)

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Citer

Thi Mong Diep Nguyen, Laura Filliatreau, Danièle Klett, Nong Van Hai, Nguyen Thuy Duong, et al.. Effect of soluble adenylyl cyclase (ADCY10) inhibitors on the LH-stimulated cAMP synthesis in Mltc-1 leydig cell line. International Journal of Molecular Sciences, 2021, 22 (9), pp.1-14. ⟨10.3390/ijms22094641⟩. ⟨hal-03468377⟩
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