Chromatin interaction of TATA-binding protein is dynamically regulated in human cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Cell Science Année : 2010

Chromatin interaction of TATA-binding protein is dynamically regulated in human cells

Résumé

Gene transcription in mammalian cells is a dynamic process involving regulated assembly of transcription complexes on chromatin in which the TATA-binding protein (TBP) plays a central role. Here, we investigate the dynamic behaviour of TBP by a combination of fluorescence recovery after photobleaching (FRAP) and biochemical assays using human cell lines of different origin. The majority of nucleoplasmic TBP and other TFIID subunits associate with chromatin in a highly dynamic manner. TBP dynamics are regulated by the joint action of the SNF2-related BTAF1 protein and the NC2 complex. Strikingly, both BTAF1 and NC2 predominantly affect TBP dissociation rates, leaving the association rate unchanged. Chromatin immunoprecipitation shows that BTAF1 negatively regulates TBP and NC2 binding to active promoters. Our results support a model for a BTAF1-mediated release of TBP-NC2 complexes from chromatin.

Dates et versions

hal-03465203 , version 1 (03-12-2021)

Identifiants

Citer

Petra de Graaf, Florence Mousson, Bart Geverts, Elisabeth Scheer, Laszlo Tora, et al.. Chromatin interaction of TATA-binding protein is dynamically regulated in human cells. Journal of Cell Science, 2010, 123 (15), pp.2663-2671. ⟨10.1242/jcs.064097⟩. ⟨hal-03465203⟩
6 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More