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Article Dans Une Revue Journal of Cellular and Molecular Medicine Année : 2019

The toxic effect of cytostatics on primary cilia frequency and multiciliation

G. Dayanithi
Alžběta Filipová
  • Fonction : Auteur
Daniel Diaz Garcia
  • Fonction : Auteur
Aleš Bezrouk
  • Fonction : Auteur
Dana Čížková
  • Fonction : Auteur
Josef Dvořák
  • Fonction : Auteur
Stanislav Filip
Justin Sturge
  • Fonction : Auteur
Zuzana Šinkorová
  • Fonction : Auteur

Résumé

The primary cilium is considered as a key component of morphological cellular stabil-ity. However, cancer cells are notorious for lacking primary cilia in most cases, de-pending upon the tumour type. Previous reports have shown the effect of starvation and cytostatics on ciliogenesis in normal and cancer cells although with limited suc-cess, especially when concerning the latter. In this study, we evaluated the presence and frequency of primary cilia in breast fibroblasts and in triple-negative breast can-cer cells after treatment with cytostatics finding that, in the case of breast fibro-blasts, primary cilia were detected at their highest incidence 72 hours after treatment with 120 nM doxorubicin. Further, multiciliated cells were also detected after treat-ment with 80 nM doxorubicin. On the other hand, treatment with taxol increased the number of ciliated cells only at low concentrations (1.25 and 3.25 nM) and did not induce multiciliation. Interestingly, triple-negative breast cancer cells did not present primary cilia after treatment with either doxorubicin or taxol. This is the first study reporting the presence of multiple primary cilia in breast fibroblasts induced by doxo-rubicin. However, the null effect of these cytostatics on primary cilia incidence in the evaluated triple negative breast carcinomas cell lines requires further research.
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hal-03457286 , version 1 (08-06-2022)

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G. Dayanithi, Alžběta Filipová, Daniel Diaz Garcia, Aleš Bezrouk, Dana Čížková, et al.. The toxic effect of cytostatics on primary cilia frequency and multiciliation. Journal of Cellular and Molecular Medicine, 2019, 23 (8), pp.5728-5736. ⟨10.1111/jcmm.14487⟩. ⟨hal-03457286⟩
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