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Article Dans Une Revue Molecular and Cellular Biology Année : 2006

Independent Recruitment of Mediator and SAGA by the Activator Met4

Christophe Leroy
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Laëtitia Cormier
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Laurent Kuras
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Résumé

Mediator is a key RNA polymerase II (Pol II) cofactor in the regulation of eukaryotic gene expression. It is believed to function as a coactivator linking gene-specific activators to the basal Pol II initiation machinery. In support of this model, we provide evidence that Mediator serves in vivo as a coactivator for the yeast activator Met4, which controls the gene network responsible for the biosynthesis of sulfur-containing amino acids and S-adenosylmethionine. In addition, we show that SAGA (Spt-Ada-Gcn5-acetyltransferase) is also recruited to Met4 target promoters, where it participates in the recruitment of Pol II by a mechanism involving histone acetylation. Interestingly, we find that SAGA is not required for Mediator recruitment by Met4 and vice versa. Our results provide a novel example of functional interplay between Mediator and coactivators involved in histone modification.
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Dates et versions

hal-03448837 , version 1 (05-01-2022)

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Christophe Leroy, Laëtitia Cormier, Laurent Kuras. Independent Recruitment of Mediator and SAGA by the Activator Met4. Molecular and Cellular Biology, 2006, 26, pp.3149 - 3163. ⟨10.1128/mcb.26.8.3149-3163.2006⟩. ⟨hal-03448837⟩
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