<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-03445292</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-17T16:38:43+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Citrullination of pyruvate kinase M2 by PADI1 and PADI3 regulates glycolysis and cancer cell proliferation</title>
            <author role="aut">
              <persName>
                <forename type="first">Sébastien</forename>
                <surname>Coassolo</surname>
              </persName>
              <email type="md5">bc77937b7701b10a6c2ecd7a9694443b</email>
              <email type="domain">unistra.fr</email>
              <idno type="idhal" notation="numeric">1135376</idno>
              <idno type="halauthorid" notation="string">2345925-1135376</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-3669-8650</idno>
              <idno type="IDREF">https://www.idref.fr/241520622</idno>
              <affiliation ref="#struct-93800"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Guillaume</forename>
                <surname>Davidson</surname>
              </persName>
              <idno type="idhal" notation="numeric">798306</idno>
              <idno type="halauthorid" notation="string">1709651-798306</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-9272-3924</idno>
              <idno type="IDREF">https://www.idref.fr/273066218</idno>
              <affiliation ref="#struct-93800"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Luc</forename>
                <surname>Negroni</surname>
              </persName>
              <email type="md5">6da7b0fffca520e3915103e03f94fa27</email>
              <email type="domain">unistra.fr</email>
              <idno type="idhal" notation="numeric">758324</idno>
              <idno type="halauthorid" notation="string">219424-758324</idno>
              <idno type="IDREF">https://www.idref.fr/177123869</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-6204-3917</idno>
              <idno type="VIAF">https://viaf.org/viaf/307509932</idno>
              <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=3vONHOYAAAAJ</idno>
              <affiliation ref="#struct-93800"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Giovanni</forename>
                <surname>Gambi</surname>
              </persName>
              <email type="md5">75efb689bc4dec1073d262a7fb03509f</email>
              <email type="domain">unistra.fr</email>
              <idno type="idhal" notation="numeric">1135374</idno>
              <idno type="halauthorid" notation="string">1709652-1135374</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-2318-0003</idno>
              <idno type="IDREF">https://www.idref.fr/262823306</idno>
              <affiliation ref="#struct-93800"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Sylvain</forename>
                <surname>Daujat</surname>
              </persName>
              <email type="md5">c46097c51cba1dde318ff1a5ad1e7abc</email>
              <email type="domain">unistra.fr</email>
              <idno type="idhal" notation="numeric">1134657</idno>
              <idno type="halauthorid" notation="string">213324-1134657</idno>
              <idno type="IDREF">https://www.idref.fr/250394758</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-5107-119X</idno>
              <affiliation ref="#struct-93800"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Christophe</forename>
                <surname>Romier</surname>
              </persName>
              <email type="md5">9296951a66f9f9207114c162bcb19d72</email>
              <email type="domain">igbmc.fr</email>
              <idno type="idhal" notation="string">christophe-romier</idno>
              <idno type="idhal" notation="numeric">750418</idno>
              <idno type="halauthorid" notation="string">4932-750418</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-3680-935X</idno>
              <idno type="IDREF">https://www.idref.fr/161229859</idno>
              <affiliation ref="#struct-93800"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Irwin</forename>
                <surname>Davidson</surname>
              </persName>
              <email type="md5">33f247322d29b5018c6293feaf35eafb</email>
              <email type="domain">igbmc.fr</email>
              <idno type="idhal" notation="string">irwin-davidson</idno>
              <idno type="idhal" notation="numeric">742111</idno>
              <idno type="halauthorid" notation="string">5263-742111</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-5533-1171</idno>
              <idno type="IDREF">https://www.idref.fr/075977249</idno>
              <affiliation ref="#struct-93800"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Irwin</forename>
                <surname>Davidson</surname>
              </persName>
              <email type="md5">33f247322d29b5018c6293feaf35eafb</email>
              <email type="domain">igbmc.fr</email>
            </editor>
            <funder ref="#projanr-55006"/>
            <funder ref="#projanr-37796"/>
            <funder ref="#projanr-49191"/>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2021-11-23 21:13:31</date>
              <date type="whenModified">2026-01-28 10:27:42</date>
              <date type="whenReleased">2021-12-02 16:09:06</date>
              <date type="whenProduced">2021-03-19</date>
              <date type="whenEndEmbargoed">2021-11-23</date>
              <ref type="file" target="https://hal.science/hal-03445292v1/document">
                <date notBefore="2021-11-23"/>
              </ref>
              <ref type="file" subtype="greenPublisher" n="1" target="https://hal.science/hal-03445292v1/file/Coassolo%20et%20al%20Nat%20Com.pdf" id="file-3445295-3021050">
                <date notBefore="2021-11-23"/>
              </ref>
              <ref type="externalLink" target="https://www.nature.com/articles/s41467-021-21960-4.pdf"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="645221">
                <persName>
                  <forename>Irwin</forename>
                  <surname>Davidson</surname>
                </persName>
                <email type="md5">33f247322d29b5018c6293feaf35eafb</email>
                <email type="domain">igbmc.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-03445292</idno>
            <idno type="halUri">https://hal.science/hal-03445292</idno>
            <idno type="halBibtex">coassolo:hal-03445292</idno>
            <idno type="halRefHtml">&lt;i&gt;Nature Communications&lt;/i&gt;, 2021, 12 (1), &lt;a target="_blank" href="https://dx.doi.org/10.1038/s41467-021-21960-4"&gt;&amp;#x27E8;10.1038/s41467-021-21960-4&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Nature Communications, 2021, 12 (1), &amp;#x27E8;10.1038/s41467-021-21960-4&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-3445295-3021050"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="INSERM">INSERM - Institut national de la santé et de la recherche médicale</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="IGBMC" corresp="INSERM">Institut Génétique de Biologie Moléculaire et Cellulaire</idno>
            <idno type="stamp" n="UNIV-STRASBG">Université de Strasbourg</idno>
            <idno type="stamp" n="SITE-ALSACE">Archive ouverte du site Alsace</idno>
            <idno type="stamp" n="ANR">ANR</idno>
            <idno type="stamp" n="UNIVOAK">UNIVOAK</idno>
            <idno type="stamp" n="UNISTRA-ITI">Publications ITI Strasbourg</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Citrullination of pyruvate kinase M2 by PADI1 and PADI3 regulates glycolysis and cancer cell proliferation</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Sébastien</forename>
                    <surname>Coassolo</surname>
                  </persName>
                  <email type="md5">bc77937b7701b10a6c2ecd7a9694443b</email>
                  <email type="domain">unistra.fr</email>
                  <idno type="idhal" notation="numeric">1135376</idno>
                  <idno type="halauthorid" notation="string">2345925-1135376</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-3669-8650</idno>
                  <idno type="IDREF">https://www.idref.fr/241520622</idno>
                  <affiliation ref="#struct-93800"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Guillaume</forename>
                    <surname>Davidson</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">798306</idno>
                  <idno type="halauthorid" notation="string">1709651-798306</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-9272-3924</idno>
                  <idno type="IDREF">https://www.idref.fr/273066218</idno>
                  <affiliation ref="#struct-93800"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Luc</forename>
                    <surname>Negroni</surname>
                  </persName>
                  <email type="md5">6da7b0fffca520e3915103e03f94fa27</email>
                  <email type="domain">unistra.fr</email>
                  <idno type="idhal" notation="numeric">758324</idno>
                  <idno type="halauthorid" notation="string">219424-758324</idno>
                  <idno type="IDREF">https://www.idref.fr/177123869</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-6204-3917</idno>
                  <idno type="VIAF">https://viaf.org/viaf/307509932</idno>
                  <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=3vONHOYAAAAJ</idno>
                  <affiliation ref="#struct-93800"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Giovanni</forename>
                    <surname>Gambi</surname>
                  </persName>
                  <email type="md5">75efb689bc4dec1073d262a7fb03509f</email>
                  <email type="domain">unistra.fr</email>
                  <idno type="idhal" notation="numeric">1135374</idno>
                  <idno type="halauthorid" notation="string">1709652-1135374</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-2318-0003</idno>
                  <idno type="IDREF">https://www.idref.fr/262823306</idno>
                  <affiliation ref="#struct-93800"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Sylvain</forename>
                    <surname>Daujat</surname>
                  </persName>
                  <email type="md5">c46097c51cba1dde318ff1a5ad1e7abc</email>
                  <email type="domain">unistra.fr</email>
                  <idno type="idhal" notation="numeric">1134657</idno>
                  <idno type="halauthorid" notation="string">213324-1134657</idno>
                  <idno type="IDREF">https://www.idref.fr/250394758</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-5107-119X</idno>
                  <affiliation ref="#struct-93800"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Christophe</forename>
                    <surname>Romier</surname>
                  </persName>
                  <email type="md5">9296951a66f9f9207114c162bcb19d72</email>
                  <email type="domain">igbmc.fr</email>
                  <idno type="idhal" notation="string">christophe-romier</idno>
                  <idno type="idhal" notation="numeric">750418</idno>
                  <idno type="halauthorid" notation="string">4932-750418</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-3680-935X</idno>
                  <idno type="IDREF">https://www.idref.fr/161229859</idno>
                  <affiliation ref="#struct-93800"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Irwin</forename>
                    <surname>Davidson</surname>
                  </persName>
                  <email type="md5">33f247322d29b5018c6293feaf35eafb</email>
                  <email type="domain">igbmc.fr</email>
                  <idno type="idhal" notation="string">irwin-davidson</idno>
                  <idno type="idhal" notation="numeric">742111</idno>
                  <idno type="halauthorid" notation="string">5263-742111</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-5533-1171</idno>
                  <idno type="IDREF">https://www.idref.fr/075977249</idno>
                  <affiliation ref="#struct-93800"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">67823</idno>
                <idno type="issn">2041-1723</idno>
                <idno type="eissn">2041-1723</idno>
                <title level="j">Nature Communications</title>
                <imprint>
                  <publisher>Nature Publishing Group</publisher>
                  <biblScope unit="volume">12</biblScope>
                  <biblScope unit="issue">1</biblScope>
                  <date type="datePub">2021-03-19</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1038/s41467-021-21960-4</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="halDomain" n="sdv.bbm.bm">Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Abstract Chromodomain helicase DNA binding protein 4 (CHD4) is an ATPase subunit of the Nucleosome Remodelling and Deacetylation (NuRD) complex that regulates gene expression. CHD4 is essential for growth of multiple patient derived melanoma xenografts and for breast cancer. Here we show that CHD4 regulates expression of PADI1 (Protein Arginine Deiminase 1) and PADI3 in multiple cancer cell types modulating citrullination of arginine residues of the allosterically-regulated glycolytic enzyme pyruvate kinase M2 (PKM2). Citrullination of PKM2 R106 reprogrammes cross-talk between PKM2 ligands lowering its sensitivity to the inhibitors Tryptophan, Alanine and Phenylalanine and promoting activation by Serine. Citrullination thus bypasses normal physiological regulation by low Serine levels to promote excessive glycolysis and reduced cell proliferation. We further show that PADI1 and PADI3 expression is up-regulated by hypoxia where PKM2 citrullination contributes to increased glycolysis. We provide insight as to how conversion of arginines to citrulline impacts key interactions within PKM2 that act in concert to reprogramme its activity as an additional mechanism regulating this important enzyme.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-93800" status="VALID">
          <idno type="IdRef">155263749</idno>
          <idno type="ISNI">0000 0004 0638 2716</idno>
          <idno type="RNSR">200112491C</idno>
          <idno type="ROR">https://ror.org/0015ws592</idno>
          <idno type="Wikidata">Q3152004</idno>
          <orgName>Institut de Génétique et de Biologie Moléculaire et Cellulaire</orgName>
          <orgName type="acronym">IGBMC</orgName>
          <date type="start">2009-01-01</date>
          <desc>
            <address>
              <addrLine>Parc D'Innovation 1 Rue Laurent Fries - BP 10142 67404 Illkirch Cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.igbmc.fr/</ref>
          </desc>
          <listRelation>
            <relation name="UMRS964" active="#struct-199013" type="direct"/>
            <relation name="U1258" active="#struct-303623" type="direct"/>
            <relation name="UMR7104" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-199013" status="VALID">
          <idno type="IdRef">131056549</idno>
          <idno type="ROR">https://ror.org/00pg6eq24</idno>
          <orgName>Université de Strasbourg</orgName>
          <orgName type="acronym">UNISTRA</orgName>
          <desc>
            <address>
              <addrLine>4 rue Blaise Pascal - CS 90032 - 67081 Strasbourg cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.unistra.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-303623" status="VALID">
          <idno type="IdRef">026388278</idno>
          <idno type="ROR">https://ror.org/02vjkv261</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>
            <address>
              <addrLine>101, rue de Tolbiac, 75013 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
      </listOrg>
      <listOrg type="projects">
        <org type="anrProject" xml:id="projanr-55006" status="VALID">
          <idno type="anr">ANR-10-LABX-0030</idno>
          <orgName>INRT</orgName>
          <desc>Integrative Biology : Nuclear dynamics- Regenerative medicine - Translational medicine</desc>
          <date type="start">2010</date>
        </org>
        <org type="anrProject" xml:id="projanr-37796" status="VALID">
          <idno type="anr">ANR-10-IDEX-0002</idno>
          <idno type="program">Initiative d'excellence</idno>
          <orgName>UNISTRA</orgName>
          <desc>Par-delà les frontières, l'Université de Strasbourg</desc>
          <date type="start">2010</date>
        </org>
        <org type="anrProject" xml:id="projanr-49191" status="VALID">
          <idno type="anr">ANR-10-INBS-0009</idno>
          <orgName>France Génomique</orgName>
          <desc>Organisation et montée en puissance d'une Infrastructure Nationale de Génomique</desc>
          <date type="start">2010</date>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>