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Article Dans Une Revue Biosensors and Bioelectronics Année : 2021

A mask-based diagnostic platform for point-of-care screening of Covid-19

Résumé

Diagnostics of SARS-CoV-2 infection using real-time reverse-transcription polymerase chain reaction (RT-PCR) on nasopharyngeal swabs is now well-established, with saliva-based testing being lately more widely implemented for being more adapted for self-testing approaches. In this study, we introduce a different concept based on exhaled breath condensate (EBC), readily collected by a mask-based sampling device, and detection with an electrochemical biosensor with a modular architecture that enables fast and specific detection and quantification of COVID-19. The face mask forms an exhaled breath vapor containment volume to hold the exhaled breath vapor in proximity to the EBC collector to enable a condensate-forming surface, cooled by a thermal mass, to coalesce the exhaled breath into a 200–500 μL fluid sample in 2 min. EBC RT-PCR for SARS-CoV-2 genes (E, ORF1ab) on samples collected from 7 SARS-CoV-2 positive and 7 SARS-CoV-2 negative patients were performed. The presence of SARS-CoV-2 could be detected in 5 out of 7 SARS-CoV-2 positive patients. Furthermore, the EBC samples were screened on an electrochemical aptamer biosensor, which detects SARS-CoV-2 viral particles down to 10 pfu mL−1 in cultured SARS-CoV-2 suspensions. Using a “turn off” assay via ferrocenemethanol redox mediator, results about the infectivity state of the patient are obtained in 10 min.
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Dates et versions

hal-03442228 , version 1 (02-08-2023)

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Paternité - Pas d'utilisation commerciale

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John Daniels, Shekhar Wadekar, Ken Decubellis, George Jackson, Alexander Chiu, et al.. A mask-based diagnostic platform for point-of-care screening of Covid-19. Biosensors and Bioelectronics, 2021, 192, pp.113486. ⟨10.1016/j.bios.2021.113486⟩. ⟨hal-03442228⟩
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