<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-03438550</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-17T12:58:58+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Loss of LRP1 promotes acquisition of contractile-myofibroblast phenotype and release of active TGF-β1 from ECM stores</title>
            <author role="aut">
              <persName>
                <forename type="first">Jennifer</forename>
                <surname>Schnieder</surname>
              </persName>
              <idno type="idhal" notation="numeric">815380</idno>
              <idno type="halauthorid" notation="string">2343179-815380</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-5278-5012</idno>
              <affiliation ref="#struct-1049461"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Argen</forename>
                <surname>Mamazhakypov</surname>
              </persName>
              <idno type="halauthorid">2343180-0</idno>
              <affiliation ref="#struct-1049461"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Anna</forename>
                <surname>Birnhuber</surname>
              </persName>
              <idno type="halauthorid">2343150-0</idno>
              <affiliation ref="#struct-439573"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Jochen</forename>
                <surname>Wilhelm</surname>
              </persName>
              <idno type="idhal" notation="numeric">799197</idno>
              <idno type="halauthorid" notation="string">1751313-799197</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-5544-9647</idno>
              <affiliation ref="#struct-1049461"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Grazyna</forename>
                <surname>Kwapiszewska</surname>
              </persName>
              <idno type="halauthorid">1751315-0</idno>
              <affiliation ref="#struct-439573"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Clemens</forename>
                <surname>Ruppert</surname>
              </persName>
              <idno type="halauthorid">2343181-0</idno>
              <affiliation ref="#struct-1049461"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Philipp</forename>
                <surname>Markart</surname>
              </persName>
              <idno type="halauthorid">2343128-0</idno>
              <affiliation ref="#struct-303418"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Lukasz</forename>
                <surname>Wujak</surname>
              </persName>
              <idno type="halauthorid">2343175-0</idno>
              <affiliation ref="#struct-1049461"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Karla</forename>
                <surname>Rubio</surname>
              </persName>
              <email type="md5">05b26a71722deca456ca236ec9c09d2b</email>
              <email type="domain">univ-lorraine.fr</email>
              <idno type="idhal" notation="numeric">815358</idno>
              <idno type="halauthorid" notation="string">2343102-815358</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-8337-3106</idno>
              <affiliation ref="#struct-527097"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Guillermo</forename>
                <surname>Barreto</surname>
              </persName>
              <email type="md5">a2f06468f73c8786dd9b5f4bde53e0b5</email>
              <email type="domain">univ-lorraine.fr</email>
              <idno type="idhal" notation="string">guillermo-barreto</idno>
              <idno type="idhal" notation="numeric">749236</idno>
              <idno type="halauthorid" notation="string">54826-749236</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-7777-4712</idno>
              <affiliation ref="#struct-237046"/>
              <affiliation ref="#struct-527097"/>
              <affiliation ref="#struct-22789"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Liliana</forename>
                <surname>Schaefer</surname>
              </persName>
              <idno type="idhal" notation="numeric">808050</idno>
              <idno type="halauthorid" notation="string">597734-808050</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-3318-3005</idno>
              <affiliation ref="#struct-91203"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Malgorzata</forename>
                <surname>Wygrecka</surname>
              </persName>
              <idno type="idhal" notation="numeric">762581</idno>
              <idno type="halauthorid" notation="string">457661-762581</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-3656-2932</idno>
              <affiliation ref="#struct-1049461"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Guillermo</forename>
                <surname>Barreto</surname>
              </persName>
              <email type="md5">a2f06468f73c8786dd9b5f4bde53e0b5</email>
              <email type="domain">univ-lorraine.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2021-11-21 19:00:05</date>
              <date type="whenModified">2025-06-18 08:34:09</date>
              <date type="whenReleased">2021-11-21 19:00:05</date>
              <date type="whenProduced">2020-06</date>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="969069">
                <persName>
                  <forename>Guillermo</forename>
                  <surname>Barreto</surname>
                </persName>
                <email type="md5">a2f06468f73c8786dd9b5f4bde53e0b5</email>
                <email type="domain">univ-lorraine.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-03438550</idno>
            <idno type="halUri">https://hal.science/hal-03438550</idno>
            <idno type="halBibtex">schnieder:hal-03438550</idno>
            <idno type="halRefHtml">&lt;i&gt;Matrix Biology&lt;/i&gt;, 2020, 88, pp.69-88. &lt;a target="_blank" href="https://dx.doi.org/10.1016/j.matbio.2019.12.001"&gt;&amp;#x27E8;10.1016/j.matbio.2019.12.001&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Matrix Biology, 2020, 88, pp.69-88. &amp;#x27E8;10.1016/j.matbio.2019.12.001&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UPEC" corresp="CV_UPEC">Université Paris-Est Créteil Val-de-Marne</idno>
            <idno type="stamp" n="CRRET" corresp="UPEC">Laboratoire de recherche sur la croissance cellulaire, la réparation et la régénération tissulaires</idno>
            <idno type="stamp" n="UNIV-LORRAINE">Université de Lorraine</idno>
            <idno type="stamp" n="IMOPA-UL">IMoPA - Ingénierie Moléculaire, Cellulaire et Physiopathologie</idno>
            <idno type="stamp" n="TEST-HALCNRS">Collection test HAL CNRS</idno>
            <idno type="stamp" n="BMS-UL">Pôle scientifique Biologie, Médecine, Santé de l'Université de Lorraine</idno>
            <idno type="stamp" n="IMOPA-EQ2">Ingénierie Moléculaire, Cellulaire, Thérapeutique &amp; Glycosyltransférases (MolCelTEG)</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Loss of LRP1 promotes acquisition of contractile-myofibroblast phenotype and release of active TGF-β1 from ECM stores</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Jennifer</forename>
                    <surname>Schnieder</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">815380</idno>
                  <idno type="halauthorid" notation="string">2343179-815380</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-5278-5012</idno>
                  <affiliation ref="#struct-1049461"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Argen</forename>
                    <surname>Mamazhakypov</surname>
                  </persName>
                  <idno type="halauthorid">2343180-0</idno>
                  <affiliation ref="#struct-1049461"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Anna</forename>
                    <surname>Birnhuber</surname>
                  </persName>
                  <idno type="halauthorid">2343150-0</idno>
                  <affiliation ref="#struct-439573"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Jochen</forename>
                    <surname>Wilhelm</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">799197</idno>
                  <idno type="halauthorid" notation="string">1751313-799197</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-5544-9647</idno>
                  <affiliation ref="#struct-1049461"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Grazyna</forename>
                    <surname>Kwapiszewska</surname>
                  </persName>
                  <idno type="halauthorid">1751315-0</idno>
                  <affiliation ref="#struct-439573"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Clemens</forename>
                    <surname>Ruppert</surname>
                  </persName>
                  <idno type="halauthorid">2343181-0</idno>
                  <affiliation ref="#struct-1049461"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Philipp</forename>
                    <surname>Markart</surname>
                  </persName>
                  <idno type="halauthorid">2343128-0</idno>
                  <affiliation ref="#struct-303418"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Lukasz</forename>
                    <surname>Wujak</surname>
                  </persName>
                  <idno type="halauthorid">2343175-0</idno>
                  <affiliation ref="#struct-1049461"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Karla</forename>
                    <surname>Rubio</surname>
                  </persName>
                  <email type="md5">05b26a71722deca456ca236ec9c09d2b</email>
                  <email type="domain">univ-lorraine.fr</email>
                  <idno type="idhal" notation="numeric">815358</idno>
                  <idno type="halauthorid" notation="string">2343102-815358</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-8337-3106</idno>
                  <affiliation ref="#struct-527097"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Guillermo</forename>
                    <surname>Barreto</surname>
                  </persName>
                  <email type="md5">a2f06468f73c8786dd9b5f4bde53e0b5</email>
                  <email type="domain">univ-lorraine.fr</email>
                  <idno type="idhal" notation="string">guillermo-barreto</idno>
                  <idno type="idhal" notation="numeric">749236</idno>
                  <idno type="halauthorid" notation="string">54826-749236</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-7777-4712</idno>
                  <affiliation ref="#struct-237046"/>
                  <affiliation ref="#struct-527097"/>
                  <affiliation ref="#struct-22789"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Liliana</forename>
                    <surname>Schaefer</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">808050</idno>
                  <idno type="halauthorid" notation="string">597734-808050</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-3318-3005</idno>
                  <affiliation ref="#struct-91203"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Malgorzata</forename>
                    <surname>Wygrecka</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">762581</idno>
                  <idno type="halauthorid" notation="string">457661-762581</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-3656-2932</idno>
                  <affiliation ref="#struct-1049461"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">16944</idno>
                <idno type="issn">0945-053X</idno>
                <idno type="eissn">1569-1802</idno>
                <title level="j">Matrix Biology</title>
                <imprint>
                  <publisher>Elsevier</publisher>
                  <biblScope unit="volume">88</biblScope>
                  <biblScope unit="pp">69-88</biblScope>
                  <date type="datePub">2020-06</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1016/j.matbio.2019.12.001</idno>
              <idno type="pubmed">31841706</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">Contractility</term>
                <term xml:lang="en">Extracellular matrix</term>
                <term xml:lang="en">Fibrosis</term>
                <term xml:lang="en">Low-density lipoprotein receptor-related protein 1</term>
                <term xml:lang="en">Phenotypic switch</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>In healing tissue, fibroblasts differentiate to α-smooth muscle actin (SMA)-expressing contractile-myofibroblasts, which pull the wound edges together ensuring proper tissue repair. Uncontrolled expansion of the myofibroblast population may, however, lead to excessive tissue scarring and finally to organ dysfunction. Here, we demonstrate that the loss of low-density lipoprotein receptor-related protein (LRP) 1 overactivates the JNK1/2-c-Jun-Fra-2 signaling pathway leading to the induction of α-SMA and periostin expression in human lung fibroblasts (hLF). These changes are accompanied by increased contractility of the cells and the integrin- and protease-dependent release of active transforming growth factor (TGF)-β1 from the extracellular matrix (ECM) stores. Liberation of active TGF-β1 from the ECM further enhances α-SMA and periostin expression thus accelerating the phenotypic switch of hLF. Global gene expression profiling of LRP1-depleted hLF revealed that the loss of LRP1 affects cytoskeleton reorganization, cell-ECM contacts, and ECM production. In line with these findings, fibrotic changes in the skin and lung of Fra-2 transgenic mice were associated with LRP1 depletion and c-Jun overexpression. Altogether, our results suggest that dysregulation of LRP1 expression in fibroblasts in healing tissue may lead to the unrestrained expansion of contractile myofibroblasts and thereby to fibrosis development. Further studies identifying molecules, which regulate LRP1 expression, may provide new therapeutic options for largely untreatable human fibrotic diseases.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="institution" xml:id="struct-1049461" status="INCOMING">
          <orgName>University of Giessen and Marburg Lung Center [Gießen, Germany]</orgName>
          <orgName type="acronym">UGMLC</orgName>
          <desc>
            <address>
              <country key="DE"/>
            </address>
          </desc>
        </org>
        <org type="institution" xml:id="struct-439573" status="VALID">
          <idno type="ROR">https://ror.org/009r5p347</idno>
          <orgName>Ludwig Boltzmann Institute for Lung Vascular Research [Graz]</orgName>
          <desc>
            <address>
              <addrLine>Stiftingtalstrasse 248010 Graz</addrLine>
              <country key="AT"/>
            </address>
            <ref type="url">http://lvr.lbg.ac.at/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-303418" status="VALID">
          <orgName>Philipps Universität Marburg = Philipps University of Marburg</orgName>
          <date type="start">1527-01-01</date>
          <desc>
            <address>
              <addrLine>Philipps-Universität Marburg, Biegenstraße 10, 35032 Marburg</addrLine>
              <country key="DE"/>
            </address>
            <ref type="url">https://www.uni-marburg.de/de</ref>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-527097" status="VALID">
          <orgName>Max Planck Institute for Heart and Lung Research</orgName>
          <orgName type="acronym">MPI-HLR</orgName>
          <desc>
            <address>
              <addrLine>Ludwigstr. 43, 61231 Bad Nauheim</addrLine>
              <country key="DE"/>
            </address>
            <ref type="url">https://www.mpg.de/149809/herz_lungenforschung</ref>
          </desc>
          <listRelation>
            <relation active="#struct-5247" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-237046" status="OLD">
          <idno type="IdRef">180727281</idno>
          <idno type="ISNI">0000000417589034</idno>
          <idno type="RNSR">201320578R</idno>
          <idno type="IdUnivLorraine">[UL]RNG--</idno>
          <idno type="ROR">https://ror.org/04yc2e502</idno>
          <orgName>Ingénierie Moléculaire et Physiopathologie Articulaire</orgName>
          <orgName type="acronym">IMoPA</orgName>
          <date type="start">2013-01-01</date>
          <date type="end">2023-12-31</date>
          <desc>
            <address>
              <addrLine>Université de Lorraine, Faculté de Médicine, 9 avenue de la Forêt de Haye, BP 184, 54505 Vandoeuvre-les-Nancy Cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.imopa.cnrs.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-413289" type="direct"/>
            <relation name="UMR7365" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-22789" status="OLD">
          <idno type="IdRef">158987837</idno>
          <idno type="RNSR">200918516S</idno>
          <orgName>Croissance cellulaire, réparation et régénération tissulaires</orgName>
          <orgName type="acronym">CRRET</orgName>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <addrLine>UPEC - Faculté des sciences de technologie  61 Av du général de Gaulle 94010 CRETEIL cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.vjf.cnrs.fr/spip/crret/</ref>
          </desc>
          <listRelation>
            <relation name="EA4397" active="#struct-420786" type="direct"/>
            <relation name="ERL9215 / EAC7149 / UMR7149" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-91203" status="VALID">
          <idno type="ISNI">0000 0004 1936 9721</idno>
          <idno type="ROR">https://ror.org/04cvxnb49</idno>
          <orgName>Goethe University Frankfurt = Goethe-Universität Frankfurt am Main</orgName>
          <date type="start">1914-01-01</date>
          <desc>
            <address>
              <addrLine>Theodor-W.-Adorno-Platz 1 , 60323 Frankfurt am Main</addrLine>
              <country key="DE"/>
            </address>
            <ref type="url">http://www.goethe-university-frankfurt.de/en?</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-5247" status="VALID">
          <orgName>Max-Planck-Gesellschaft</orgName>
          <desc>
            <address>
              <addrLine>Jägerstrasse, 10-11, D-10117 Berlin</addrLine>
              <country key="DE"/>
            </address>
            <ref type="url">https://www.mpg.de/en</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-413289" status="VALID">
          <idno type="IdRef">157040569</idno>
          <idno type="IdUnivLorraine">[UL]100--</idno>
          <idno type="ROR">https://ror.org/04vfs2w97</idno>
          <orgName>Université de Lorraine</orgName>
          <orgName type="acronym">UL</orgName>
          <date type="start">2012-01-01</date>
          <desc>
            <address>
              <addrLine>34 cours Léopold - CS 25233 - 54052 Nancy cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-lorraine.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-420786" status="VALID">
          <idno type="IdRef">028021037</idno>
          <idno type="ISNI">0000000121497878</idno>
          <idno type="ROR">https://ror.org/05ggc9x40</idno>
          <orgName>Université Paris-Est Créteil Val-de-Marne - Paris 12</orgName>
          <orgName type="acronym">UPEC UP12</orgName>
          <date type="start">1970-01-01</date>
          <desc>
            <address>
              <addrLine>61 avenue du Général de Gaulle - 94010 Créteil cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.u-pec.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>