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Article Dans Une Revue Microbiology Spectrum Année : 2021

Similar Methanogenic Shift but Divergent Syntrophic Partners in Anaerobic Digesters Exposed to Direct versus Successive Ammonium Additions

Résumé

During anaerobic digestion (AD) of protein-rich wastewater, ammonium (NH41) is released by amino acid degradation. High NH41 concentrations disturb the AD microbiome balance, leading to process impairments. The sensitivity of the AD microbiome to NH41 and the inhibition threshold depend on multiple parameters, especially the previ-ous microbial acclimation to ammonium stress. However, little is known about the effect of different NH41 acclimation strategies on the differential expression of key active micro-bial taxa. Here, we applied NH41 inputs of increasing intensity (from 1.7 to 15.2 g N-NH41liters21) in batch assays fed with synthetic wastewater, according to two different strat-egies: (i) direct independent inputs at a unique target concentration and (ii) successive inputs in a stepwise manner. In both strategies, along the NH41 gradient, the active metha-nogens shifted from acetoclastic Methanosaeta to Methanosarcina and eventually hydroge-notrophic Methanoculleus. Despite shorter latency times, the successive input modality led to lower methane production rate, lower soluble chemical oxygen demand (sCOD) removal efficiency, and lower half maximal inhibitory concentration, together with higher volatile fatty acid (VFA) accumulation, compared to the independent input modality. These differ-ential performances were associated with a drastically distinct succession pattern of the active bacterial partners in both experiments. In particular, the direct exposure modality was characterized by a progressive enrichment of VFA producers (mainly Tepidimicrobium) and syntrophic VFA oxidizers (mainly Syntrophaceticus) with increasing NH41 concentration, while the successive exposure modality was characterized by a more dynamic succession of VFA producers (mainly Clostridium, Sporanaerobacter, Terrisporobacter) and syntrophic VFA oxidizers (mainly Tepidanaerobacter, Syntrophomonas). These results bring relevant insights for improved process management through inoculum adaptation, bioaugmenta-tion, or community-driven optimization.
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hal-03435672 , version 1 (18-11-2021)

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Julie Hardy, Patricia Bonin, Adele Lazuka, Estelle Gonidec, Sophie Guasco, et al.. Similar Methanogenic Shift but Divergent Syntrophic Partners in Anaerobic Digesters Exposed to Direct versus Successive Ammonium Additions. Microbiology Spectrum, 2021, 9 (2), ⟨10.1128/Spectrum.00805-21⟩. ⟨hal-03435672⟩
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