Transdermal Delivery of Macromolecules Using Two-in-One Nanocomposite Device for Skin Electroporation - Archive ouverte HAL Access content directly
Journal Articles Pharmaceutics Year : 2021

Transdermal Delivery of Macromolecules Using Two-in-One Nanocomposite Device for Skin Electroporation

Juliette Simon
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  • PersonId : 1116596
Bastien Jouanmiqueou
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  • PersonId : 1116597
Marie-Pierre Rols
Muriel Golzio
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  • PersonId : 1059746

Abstract

Delivery of hydrophilic molecules through the skin using electroporation is a promising alternative approach to intradermal injection. Recently, we developed a two-in-one electrode/reservoir material composed of carbon nanotubes and agarose hydrogel. In this work, we evaluated the potential of the device to achieve non-invasive transdermal drug delivery using skin electroporation. As it involved an electrode configuration different from the literature, critical questions were raised. First, we demonstrated the efficiency of the device to permeabilize the skin of hairless mice, as observed by propidium iodide (PI) uptake in the nuclei of the epidermis cells through macro fluorescence imaging and histology. Application of Lucifer yellow (LY) at different times after unipolar electroporation treatment demonstrated the partial reversibility of the skin permeabilization after 30 min, and as such, that barrier function properties tended to be restored. We uncovered, for the first time to our knowledge, an intrinsic asymmetry of permeation pathways generated in the stratum corneum during treatment. Electrophoresis was here the main driving force for macromolecule delivery, but it competed with passive diffusion through the generated aqueous pathways for smaller molecules. Finally, we validated 4 kDa dextran labelled with fluorescein isothiocyanate (FD4) as a model molecule to optimize the electrical parameters, needed to improve macromolecule delivery.
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hal-03427408 , version 1 (13-11-2021)

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Juliette Simon, Bastien Jouanmiqueou, Marie-Pierre Rols, Emmanuel Flahaut, Muriel Golzio. Transdermal Delivery of Macromolecules Using Two-in-One Nanocomposite Device for Skin Electroporation. Pharmaceutics, 2021, 13 (11), pp.1805. ⟨10.3390/pharmaceutics13111805⟩. ⟨hal-03427408⟩
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