Repurposing the Hybrid Capture 2 (HC2) screening test for whole-genome sequencing of human papillomaviruses - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Archives of Virology Année : 2021

Repurposing the Hybrid Capture 2 (HC2) screening test for whole-genome sequencing of human papillomaviruses

Résumé

Simple and standardized approaches for genome analysis of human papillomavirus (HPV) by next-generation sequencing are needed. The aim of the study was to develop a protocol for direct deep sequencing of high-risk (hr) HPV strains, based on the widely used commercial Hybrid Capture 2 (QIAGEN) test, without any additional probe design. This protocol was applied to 15 HPV-positive and two HPV-negative cervical samples or cell lines and validated at the genotype level by comparing the sequencing results to those obtained using a commercial genotyping kit. The performance of our protocol, presented in this proof-of-principle study, supports its use for accurate characterization of genetic variants of hrHPV.
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Dates et versions

hal-03425708 , version 1 (04-10-2022)

Identifiants

Citer

A. Debernardi, W. Jarassier, C. Soret, C. Mougin, Samuel Alizon, et al.. Repurposing the Hybrid Capture 2 (HC2) screening test for whole-genome sequencing of human papillomaviruses. Archives of Virology, 2021, 166 (12), pp.3421-3425. ⟨10.1007/s00705-021-05259-9⟩. ⟨hal-03425708⟩
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