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Communication Dans Un Congrès Année : 2015

Development and validation of a bioartificial liver device with fluidized bed bioreactors hosting alginate-encapsulated hepatocyte spheroids

Ulysse Pereira
H. Rada
  • Fonction : Auteur
N. Semenzato
  • Fonction : Auteur
D. Pouchoulin
  • Fonction : Auteur
C. Legallais

Résumé

Acute and acute-on-chronic liver failure are associated to high mortality when transplantation is not possible. The lack of donors has resulted in an important demand for liver support devices. This paper describes the design and validation of a new bioartificial liver (BAL) device including fluidized bed bioreactors hosting alginate-encapsulated hepatocytes spheroids. To ensure the efficacy of the BAL and the safety of the patients, a complex extracorporeal circulation was designed to be compatible with a commercial medical device, the Prismaflex ® monitor, already used in intensive care units. Preclinical studies on large animal show that the treatment was well tolerated in terms of hemodynamics considerations. A method using non adhesive coating in petri dish led to the production of large amount of viable spheroids in vitro that were further encapsulated to follow up bioartificial liver activity during four days.
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Dates et versions

hal-03411623 , version 1 (02-11-2021)

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S. Figaro, Ulysse Pereira, H. Rada, N. Semenzato, D. Pouchoulin, et al.. Development and validation of a bioartificial liver device with fluidized bed bioreactors hosting alginate-encapsulated hepatocyte spheroids. 2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC), Aug 2015, Milan, France. pp.1335-1338, ⟨10.1109/embc.2015.7318615⟩. ⟨hal-03411623⟩
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