Mass Spectrometry of Nucleic Acid Noncovalent Complexes - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Chemical Reviews Année : 2021

Mass Spectrometry of Nucleic Acid Noncovalent Complexes

Résumé

Nucleic acids have been among the first targets for antitumor drugs and antibiotics, and with the unveiling of new biological roles in regulation of gene expression, specific DNA and RNA structures have become very attractive targets, especially when the corresponding proteins are undruggable. Biophysical assays to test target structure and ligand binding stoichiometry, affinity, specificity and binding modes are part of the drug development pipeline. Mass spectrometry offers unique advantages as a biophysical method due to its ability to distinguish each stoichiometry present in a mixture. In addition, advanced mass spectrometry approaches (reactive probing, fragmentation techniques, ion mobility spectrometry, ion spectroscopy) provide more detailed information on the complexes. Here we review the fundamentals of mass spectrometry and all its particularities when studying non-covalent nucleic acid structures, and then review what has been learned thanks to mass spectrometry on nucleic acid structures, self-assemblies (e.g., duplexes or G-quadruplexes), and their complexes with ligands.
Fichier principal
Vignette du fichier
2100806_ChemRev_revised.pdf (21.75 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03410847 , version 1 (01-11-2021)

Identifiants

Citer

Eric Largy, Alexander König, Anirban Ghosh, Debasmita Ghosh, Sanae Benabou, et al.. Mass Spectrometry of Nucleic Acid Noncovalent Complexes. Chemical Reviews, In press, ⟨10.1021/acs.chemrev.1c00386⟩. ⟨hal-03410847⟩
183 Consultations
419 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More