Detection of the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein at Physiologically Relevant Concentrations Using Surface-Enhanced Raman Spectroscopy - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2021

Detection of the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein at Physiologically Relevant Concentrations Using Surface-Enhanced Raman Spectroscopy

Résumé

Surface-enhanced Raman scattering (SERS) spectroscopy is a surface- or cavity-enhanced variant of Raman scattering spectroscopy allowing the detection of analytes with a sensitivity down to single molecules. The main prerequisite for the effectiveness of this method is the development of SERS-active surfaces or cavities capable of concentrating incident radiation into small mode volumes containing the analyte. Here, we have demonstrated that concentration of light in an ultranarrow metal–dielectric nano-cavity between a film of the receptor-binding domain (RBD) of SARS-CoV-2 S glycoprotein and silver surface, which is formed via interaction of reduced sulfhydryl groups of the RBD with silver, allows recording SERS spectra at concentrations sufficient for ultrasensitive detection of viral protein antigens at physiologically relevant (sub-picogram) levels. Moreover, an additional increase in the cavity Q-factor upon coating the dielectric RBD film with a nanometer-thick silver shell results in an additional order(s) of magnitude enhancement of the protein SERS signal, thus ensuring a sub-femtogram sensitivity of the viral antigen detection. A simplified theoretical model explaining the observed additional enhancement of the SERS signal from the protein coated with a silver film is proposed. Our study is the first to obtain characteristic Raman and SERS spectra of the RBD of S glycoprotein, the key SARS-CoV-2 viral antigen directly, without the use of low-molecular-weight Raman-reporter molecules. The possibility of direct recording of characteristic spectra of viral protein antigens at the concentration orders of magnitude lower than those required for the detection of the whole virus in biological media makes the development of a high-performance optical method for detecting and conformational analyzing the pathogen variants a realistic task.
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Dates et versions

hal-03408225 , version 1 (29-10-2021)

Identifiants

  • HAL Id : hal-03408225 , version 1

Citer

Konstantin Mochalov, Ivan Vaskan, Andrey K Sarychev, Alexandre V Ivanov, Igor V Bykov, et al.. Detection of the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein at Physiologically Relevant Concentrations Using Surface-Enhanced Raman Spectroscopy. 2021. ⟨hal-03408225⟩

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