Electrochemical and electronic detection of biomarkers in serum: a systematic comparison using aptamer-functionalized surfaces
Vladyslav Mishyn
(1)
,
Teresa Rodrigues
(1, 2)
,
Yann R. Leroux
(3)
,
Laura Butruille
(4)
,
Eloise Woitrain
(4)
,
David Montaigne
(4)
,
Patrik Aspermair
(2)
,
Henri Happy
(1, 5)
,
Wolfgang Knoll
(2, 6)
,
Rabah Boukherroub
(1, 7)
,
Sabine Szunerits
(1, 7)
1
IEMN -
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520
2 AIT - Austrian Institute of Technology [Vienna]
3 ISCR - Institut des Sciences Chimiques de Rennes
4 RNMCD - Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U 1011
5 CARBON - IEMN - Carbon - IEMN
6 DPU - Danube Private University [Krems, Autriche]
7 NBI - IEMN - NanoBioInterfaces - IEMN
2 AIT - Austrian Institute of Technology [Vienna]
3 ISCR - Institut des Sciences Chimiques de Rennes
4 RNMCD - Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U 1011
5 CARBON - IEMN - Carbon - IEMN
6 DPU - Danube Private University [Krems, Autriche]
7 NBI - IEMN - NanoBioInterfaces - IEMN
Vladyslav Mishyn
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- PersonId : 1334952
- IdHAL : vladyslav-mishyn
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Teresa Rodrigues
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- PersonId : 1114818
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Yann R. Leroux
- Fonction : Auteur
- PersonId : 4595
- IdHAL : yann-leroux
- ORCID : 0000-0002-4737-9922
Patrik Aspermair
- Fonction : Auteur
- PersonId : 804952
- ORCID : 0000-0003-1671-1328
- IdRef : 253505658
Henri Happy
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- PersonId : 748086
- IdHAL : henri-happy
- ORCID : 0000-0003-2065-8080
- IdRef : 103891455
Wolfgang Knoll
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- PersonId : 776843
- ORCID : 0000-0003-1543-4090
- IdRef : 113981597
Rabah Boukherroub
- Fonction : Auteur
- PersonId : 177969
- IdHAL : rabah-boukherroub
- ORCID : 0000-0002-9795-9888
- IdRef : 139279474
Sabine Szunerits
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- PersonId : 21321
- IdHAL : sabine-szunerits
- ORCID : 0000-0002-1567-4943
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Résumé
Sensitive and selective detection of biomarkers in serum in a short time has a significant impact on health. The enormous clinical importance of developing reliable methods and devices for testing serum levels of cardiac troponin I (cTnI), which are directly correlated to acute myocardial infarction (AMI), has spurred an unmatched race among researchers for the development of highly sensitive and cost-effective sensing formats to be able to differentiate patients with early onset of cardiac injury from healthy individuals with a mean cTnI level of 26 pg mL-1. Electronic- and electrochemical-based detection schemes allow for fast and quantitative detection not otherwise possible at the point of care. Such approaches rely largely on voltammetric and field-effect-based readouts. Here, we systematically investigate electric and electrochemical point-of-care sensors for the detection of cTnI in serum samples by using the same surface receptors, cTnI aptamer-functionalized CVD graphene-coated interdigated gold electrodes. The analytical performances of both sensors are comparable with a limit of detection (LoD) of 5.7 ± 0.6 pg mL-1(electrochemical) and 3.3 ± 1.2 pg mL-1 (electric). However, both sensors exhibit different equilibrium dissociation constant (KD) values between the aptamer-linked surface receptor and the cTnI analyte, being 160 pg mL-1 for the electrochemical and about three times lower for the electrical approach with KD = 51.4 pg mL-1. This difference is believed to be related to the use of a redox mediator in the electrochemical sensor for readout. The ability of the redox mediator to diffuse from the solution to the surface via the cTnI/aptamer interface is hindered, correlating to higher KD values. In contrast, the electric readout has the advantage of being label-free with a sensing limitation due to ionic strength effects, which can be limited using poly(ethylene) glycol surface ligands.
Domaines
Sciences de l'ingénieur [physics]Format du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Résumé |
en
Sensitive and selective detection of biomarkers in serum in a short time has a significant impact on health. The enormous clinical importance of developing reliable methods and devices for testing serum levels of cardiac troponin I (cTnI), which are directly correlated to acute myocardial infarction (AMI), has spurred an unmatched race among researchers for the development of highly sensitive and cost-effective sensing formats to be able to differentiate patients with early onset of cardiac injury from healthy individuals with a mean cTnI level of 26 pg mL-1. Electronic- and electrochemical-based detection schemes allow for fast and quantitative detection not otherwise possible at the point of care. Such approaches rely largely on voltammetric and field-effect-based readouts. Here, we systematically investigate electric and electrochemical point-of-care sensors for the detection of cTnI in serum samples by using the same surface receptors, cTnI aptamer-functionalized CVD graphene-coated interdigated gold electrodes. The analytical performances of both sensors are comparable with a limit of detection (LoD) of 5.7 ± 0.6 pg mL-1(electrochemical) and 3.3 ± 1.2 pg mL-1 (electric). However, both sensors exhibit different equilibrium dissociation constant (KD) values between the aptamer-linked surface receptor and the cTnI analyte, being 160 pg mL-1 for the electrochemical and about three times lower for the electrical approach with KD = 51.4 pg mL-1. This difference is believed to be related to the use of a redox mediator in the electrochemical sensor for readout. The ability of the redox mediator to diffuse from the solution to the surface via the cTnI/aptamer interface is hindered, correlating to higher KD values. In contrast, the electric readout has the advantage of being label-free with a sensing limitation due to ionic strength effects, which can be limited using poly(ethylene) glycol surface ligands.
|
Titre |
en
Electrochemical and electronic detection of biomarkers in serum: a systematic comparison using aptamer-functionalized surfaces
|
Auteur(s) |
Vladyslav Mishyn
1
, Teresa Rodrigues
1, 2
, Yann R. Leroux
3
, Laura Butruille
4
, Eloise Woitrain
4
, David Montaigne
4
, Patrik Aspermair
2
, Henri Happy
1, 5
, Wolfgang Knoll
2, 6
, Rabah Boukherroub
1, 7
, Sabine Szunerits
1, 7
1
IEMN -
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520
( 1066983 )
- [Univ. Lille, CNRS, Centrale Lille Institut, Junia, Univ. Polytechnique Hauts-de-France] ––
Laboratoire Central – Cité Scientifique – Avenue Poincaré – CS 60069 – 59652 VILLENEUVE D’ASCQ CEDEX
- France
2
AIT -
Austrian Institute of Technology [Vienna]
( 196396 )
- Donau-City-Straße 1
1220 Vienna
- Autriche
3
ISCR -
Institut des Sciences Chimiques de Rennes
( 194938 )
- Campus de Beaulieu - Bât. 10 Avenue du Général Leclerc 35042 Rennes Cedex
- France
4
RNMCD -
Récepteurs Nucléaires, Maladies Métaboliques et Cardiovasculaires - U 1011
( 1059103 )
- 1 rue du Prof Calmette - 59019 LILLE Cedex
- France
5
CARBON - IEMN -
Carbon - IEMN
( 1067376 )
- Groupe Carbon - IEMN UMR8520
- France
6
DPU -
Danube Private University [Krems, Autriche]
( 1003650 )
- Autriche
7
NBI - IEMN -
NanoBioInterfaces - IEMN
( 1067477 )
- Groupe NBI - CCHB IRCICA - 50 Avenue Halley - 59650 Villeneuve d'Ascq
- France
|
Numéro |
18
|
Nom de la revue |
|
Langue du document |
Anglais
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Date de publication |
2022-07
|
Date de publication électronique |
2021-10-01
|
Volume |
414
|
Page/Identifiant |
5319–5327
|
Référence interne |
|
Domaine(s) |
|
Financement |
|
Mots-clés |
en
Aptamer, Differential pulse voltammetry, Graphene-based field-effect transistor, cTnI
|
DOI | 10.1007/s00216-021-03658-0 |
Pubmed Id | 34595559 |
UT key WOS | 000702201000005 |
Origine :
Fichiers produits par l'(les) auteur(s)
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