Article Dans Une Revue Nature Cell Biology Année : 2016

Centromeric DNA replication reconstitution reveals DNA loops and ATR checkpoint suppression

Résumé

Half of human genome is made of repetitive DNA. However, mechanisms underlying replication of chromosome regions containing repetitive DNA are poorly understood. We reconstituted replication of defined human chromosome segments using Bacterial Artificial Chromosomes (BACs) in Xenopus laevis egg extract. Using this approach we characterized chromatin assembly and replication dynamics of centromeric alpha-satellite DNA. Proteomic analysis of centromeric chromatin revealed replication dependent enrichment of a network of DNA repair factors among which the MSH2-6 complex, which was required for efficient centromeric DNA replication. However, contrary to expectations, the ATR dependent checkpoint monitoring DNA replication fork arrest could not be activated on highly repetitive DNA due to inability of single stranded DNA binding protein RPA to accumulate on chromatin. Electron microscopy of centromeric DNA and supercoil mapping revealed the presence of Topoisomerase I dependent DNA loops embedded in a protein matrix enriched for SMC2-4 proteins. This arrangement suppressed ATR signalling by preventing RPA hyper-loading, facilitating replication of centromeric DNA. These findings have important implications on our understanding of repetitive DNA metabolism and centromere organization under normal and stressful conditions.

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hal-03403448 , version 1 (26-10-2021)

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Antoine Aze, Vincenzo Sannino, Paolo Soffientini, Angela Bachi, Vincenzo Costanzo. Centromeric DNA replication reconstitution reveals DNA loops and ATR checkpoint suppression. Nature Cell Biology, 2016, 18 (6), pp.684 - 691. ⟨10.1038/ncb3344⟩. ⟨hal-03403448⟩
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