A quantitative gibberellin signalling biosensor reveals a role for gibberellins in internode specification at the shoot apical meristem - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2021

A quantitative gibberellin signalling biosensor reveals a role for gibberellins in internode specification at the shoot apical meristem

Teva Vernoux
Bihai Shi
Alejandro Navarro-Galiano
  • Fonction : Auteur
Ankit Walia
  • Fonction : Auteur
Shani Lazary
  • Fonction : Auteur
Roy Weinstein
Alexander Jones
Salomé Prat

Résumé

Growth at the shoot apical meristem (SAM) is essential for shoot architecture construction. The phytohormones gibberellins (GA) play a pivotal role in coordinating plant growth, but their role in the SAM remains mostly unknown. Here, we developed a ratiometric GA signalling biosensor by engineering one of the DELLA repressors, to suppress its master regulatory function in GA transcriptional responses while preserving its degradation upon GA sensing. We demonstrate that this novel degradation-based biosensor accurately reports on cellular changes in GA levels and perception during development. We used this biosensor to map GA signalling activity in the SAM. We show that high GA signalling is found primarily in cells located between organ primordia that are the precursors of internodes. By gain- and loss-of-function approaches, we further demonstrate that GAs regulate cell division plane orientation to establish the typical cellular organisation of internodes, thus contributing to internode specification in the SAM.

Domaines

Botanique

Dates et versions

hal-03393660 , version 1 (21-10-2021)

Identifiants

Citer

Teva Vernoux, Bihai Shi, Amelia Felipo-Benavent, Guillaume Cerutti, Carlos Galvan-Ampudia, et al.. A quantitative gibberellin signalling biosensor reveals a role for gibberellins in internode specification at the shoot apical meristem. 2021. ⟨hal-03393660⟩
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