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Article Dans Une Revue Nature Communications Année : 2021

Self-association of MreC as a regulatory signal in bacterial cell wall elongation

Résumé

Abstract The elongasome, or Rod system, is a protein complex that controls cell wall formation in rod-shaped bacteria. MreC is a membrane-associated elongasome component that co-localizes with the cytoskeletal element MreB and regulates the activity of cell wall biosynthesis enzymes, in a process that may be dependent on MreC self-association. Here, we use electron cryo-microscopy and X-ray crystallography to determine the structure of a self-associated form of MreC from Pseudomonas aeruginosa in atomic detail. MreC monomers interact in head-to-tail fashion. Longitudinal and lateral interfaces are essential for oligomerization in vitro, and a phylogenetic analysis of proteobacterial MreC sequences indicates the prevalence of the identified interfaces. Our results are consistent with a model where MreC’s ability to alternate between self-association and interaction with the cell wall biosynthesis machinery plays a key role in the regulation of elongasome activity.
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Dates et versions

hal-03369436 , version 1 (07-10-2021)

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Alexandre Martins, Carlos Contreras-Martel, Manon Janet-Maitre, Mayara Miyachiro, Leandro Estrozi, et al.. Self-association of MreC as a regulatory signal in bacterial cell wall elongation. Nature Communications, 2021, 12 (1), pp.2987. ⟨10.1038/s41467-021-22957-9⟩. ⟨hal-03369436⟩
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