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Article Dans Une Revue Molecular Biology and Evolution Année : 2019

The Oxymonad Genome Displays Canonical Eukaryotic Complexity in the Absence of a Mitochondrion

Anna Karnkowska
Sebastian Treitli
  • Fonction : Auteur
Ondřej Brzoň
  • Fonction : Auteur
Lukáš Novák
  • Fonction : Auteur
Vojtěch Vacek
  • Fonction : Auteur
Laura Eme
Petr Soukal
  • Fonction : Auteur
Lael Barlow
  • Fonction : Auteur
Emily Herman
  • Fonction : Auteur
Shweta Pipaliya
  • Fonction : Auteur
David Žihala
  • Fonction : Auteur
Romana Petrželková
  • Fonction : Auteur
Anzhelika Butenko
  • Fonction : Auteur
Courtney Stairs
Andrew Roger
Marek Eliáš
  • Fonction : Auteur
Joel Dacks
  • Fonction : Auteur
Vladimír Hampl
  • Fonction : Auteur

Résumé

Abstract The discovery that the protist Monocercomonoides exilis completely lacks mitochondria demonstrates that these organelles are not absolutely essential to eukaryotic cells. However, the degree to which the metabolism and cellular systems of this organism have adapted to the loss of mitochondria is unknown. Here, we report an extensive analysis of the M. exilis genome to address this question. Unexpectedly, we find that M. exilis genome structure and content is similar in complexity to other eukaryotes and less “reduced” than genomes of some other protists from the Metamonada group to which it belongs. Furthermore, the predicted cytoskeletal systems, the organization of endomembrane systems, and biosynthetic pathways also display canonical eukaryotic complexity. The only apparent preadaptation that permitted the loss of mitochondria was the acquisition of the SUF system for Fe–S cluster assembly and the loss of glycine cleavage system. Changes in other systems, including in amino acid metabolism and oxidative stress response, were coincident with the loss of mitochondria but are likely adaptations to the microaerophilic and endobiotic niche rather than the mitochondrial loss per se. Apart from the lack of mitochondria and peroxisomes, we show that M. exilis is a fully elaborated eukaryotic cell that is a promising model system in which eukaryotic cell biology can be investigated in the absence of mitochondria.

Dates et versions

hal-03368666 , version 1 (06-10-2021)

Identifiants

Citer

Anna Karnkowska, Sebastian Treitli, Ondřej Brzoň, Lukáš Novák, Vojtěch Vacek, et al.. The Oxymonad Genome Displays Canonical Eukaryotic Complexity in the Absence of a Mitochondrion. Molecular Biology and Evolution, 2019, 36 (10), pp.2292-2312. ⟨10.1093/molbev/msz147⟩. ⟨hal-03368666⟩
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