LptB 2 FG is an ABC transporter with Adenylate Kinase activity regulated by LptC/A recruitment - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2021

LptB 2 FG is an ABC transporter with Adenylate Kinase activity regulated by LptC/A recruitment

Résumé

ABSTRACT L ipo p oly s accharide (LPS) is an essential glycolipid covering the surface of gram-negative bacteria. Its transport involves a dedicated 7 protein transporter system, the Lpt machinery, that physically spans the entire cell envelope. LptB 2 FG complex is an ABC transporter that hydrolyses A denosine T ri p hosphate (ATP) to extract LPS from the inner membrane (IM). LptB 2 FG was extracted directly from IM with its original lipid environment by Styrene-Maleic acids polymers(SMA). SMA-LptB 2 FG in nanodiscs displays ATPase activity and a previously uncharacterized A denylate K inase (AK) activity. It catalyzes phosphotransfer between two ADP molecules to generate ATP and AMP. ATPase and AK activities of LptB 2 FG are both stimulated by the interaction on the periplasmic side with LptC and LptA partners and inhibited by the presence of LptC transmembrane helix. Isolated ATPase module (LptB) has weak AK activity in absence of LptF and LptG, and one mutation, that weakens affinity for ADP, has AK activity similar to that of fully assembled complex. LptB 2 FG is thus capable of producing ATP from ADP depending on the assembly of the Lpt bridge and the AK activity might be important to ensure efficient LPS transport in fully assembled Lpt system.

Dates et versions

hal-03365556 , version 1 (05-10-2021)

Identifiants

Citer

Tiago Baeta, Karine Giandoreggio-Barranco, Isabel Ayala, Elisabete Moura, Paola Sperandeo, et al.. LptB 2 FG is an ABC transporter with Adenylate Kinase activity regulated by LptC/A recruitment. 2021. ⟨hal-03365556⟩
22 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More