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Article Dans Une Revue Nature Communications Année : 2022

Large field-of-view non-invasive imaging through scattering layers using fluctuating random illumination

Résumé

Non-invasive optical imaging techniques are essential diagnostic tools in many fields. Although various recent methods have been proposed to utilize and control light in multiple scattering media, non-invasive optical imaging through and inside scattering layers across a large field of view remains elusive due to the physical limits set by the optical memory effect, especially without wavefront shaping techniques. Here, we demonstrate an approach that enables non-invasive fluorescence imaging behind scattering layers with field-of-views extending well beyond the optical memory effect. The method consists in demixing the speckle patterns emitted by a fluorescent object under variable unknown random illumination, using matrix factorization and a novel fingerprintbased reconstruction. Experimental validation shows the efficiency and robustness of the method with various fluorescent samples, covering a field of view up to three times the optical memory effect range. Our non-invasive imaging technique is simple, neither requires a spatial light modulator nor a guide star, and can be generalized to a wide range of incoherent contrast mechanisms and illumination schemes.
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Dates et versions

hal-03360673 , version 1 (30-09-2021)

Identifiants

Citer

Lei Zhu, Fernando Soldevila, Claudio Moretti, Alexandra d'Arco, Antoine Boniface, et al.. Large field-of-view non-invasive imaging through scattering layers using fluctuating random illumination. Nature Communications, 2022, ⟨10.1038/s41467-022-29166-y⟩. ⟨hal-03360673⟩
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