Effects of TSPO deletion on cardiac and metabolic phenotype in rats - Archive ouverte HAL
Poster De Conférence Année : 2020

Effects of TSPO deletion on cardiac and metabolic phenotype in rats

Résumé

Introduction The translocator protein (TSPO) is involved in the transport of cholesterol across mitochondrial membranes in steroidogenic tissue. TSPO is widely distributed and is associated with multiple functions, such as steroid hormones or heme synthesis. This protein is also expressed in the heart but its role remains illknown. However, our laboratory demonstrated that 4'chlorodiazepam, a TSPO ligand, reduces infarct size following ischemia-reperfusion (IR). This cardioprotection was associated with an inhibition of cholesterol intake by cardiac mitochondria and less accumulation of cholesterol oxidized derivatives. Our laboratory started a colony of rats deleted for TSPO (TSPO-/-), providing an interesting tool to further investigate the role of TSPO in cardiac IR. Objective The aim of this study is to investigate the metabolic and cardiac phenotypes of the rats deleted for TSPO. Methods TSPO+/+ and TSPO-/-rats were studied throughout their lives. The effects of the TSPO deletion on reproduction (number of pups, sexes, genotype), morphology (weight, length), cardiac phenotype (mitochondrial function, echocardiography and fibrosis), hormone production , lipid phenotype, hematologic and metabolic phenotype (insulin and glucose tolerance tests) were investigated. Results Our first results obtained in nine week old rats indicated that at young age, there was no significant differences between TSPO+/+ and TSPO-/-rats on heart rate, left ventricular ejection fraction and dimensions. Furthermore, TSPO deletion did not alter metabolic phenotype at this stage : it did not modify production nor response to insulin. Lipid profile of TSPO+/+ rats was similar to TSPO-/-. Finally we did not detect any major morphologic modification in TSPO-/-as compared to TSPO +/+ rats at this age. Conclusion Deletion of TSPO did not modify the cardiac and metabolic phenotype in young rats. The follow up of these animals will provide further data at older stages.
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Dates et versions

hal-03357977 , version 1 (01-10-2021)

Identifiants

  • HAL Id : hal-03357977 , version 1

Citer

J Bréhat, S Leick, J B Su, L Sambin, B Ghaleh, et al.. Effects of TSPO deletion on cardiac and metabolic phenotype in rats. Printemps de la cardiologie 2020, Oct 2020, Grenoble, France. ⟨hal-03357977⟩

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