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Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2021

Bacterial rhamnolipids and their 3-hydroxyalkanoate precursors activate Arabidopsis innate immunity through two independent mechanisms

Romain Schellenberger
  • Fonction : Auteur
Jérôme Crouzet
  • Fonction : Auteur
Arvin Nickzad
  • Fonction : Auteur
Lin-Jie Shu
  • Fonction : Auteur
Alexander Kutschera
  • Fonction : Auteur
Tim Gerster
  • Fonction : Auteur
Corinna Dawid
  • Fonction : Auteur
Maude Cloutier
  • Fonction : Auteur
Sandra Villaume
Sandrine Dhondt-Cordelier
Jane Hubert
  • Fonction : Auteur
Sylvain Cordelier
  • Fonction : Auteur
Florence Mazeyrat-Gourbeyre
  • Fonction : Auteur
Christian Schmid
  • Fonction : Auteur
Marc Ongena
Thomas Hofmann
  • Fonction : Auteur
Fabienne Baillieul
  • Fonction : Auteur
Christophe Clément
  • Fonction : Auteur
Cyril Zipfel
  • Fonction : Auteur
Charles Gauthier
  • Fonction : Auteur
Eric Déziel
  • Fonction : Auteur
Stefanie Ranf
  • Fonction : Auteur
Stéphan Dorey

Résumé

Plant innate immunity is activated upon perception of invasion pattern molecules by plant cell-surface immune receptors. Several bacteria of the genera Pseudomonas and Burkholderia produce rhamnolipids (RLs) from l -rhamnose and ( R )-3-hydroxyalkanoate precursors (HAAs). RL and HAA secretion is required to modulate bacterial surface motility, biofilm development, and thus successful colonization of hosts. Here, we show that the lipidic secretome from the opportunistic pathogen Pseudomonas aeruginosa , mainly comprising RLs and HAAs, stimulates Arabidopsis immunity. We demonstrate that HAAs are sensed by the bulb-type lectin receptor kinase LIPOOLIGOSACCHARIDE-SPECIFIC REDUCED ELICITATION/S-DOMAIN-1-29 (LORE/SD1-29), which also mediates medium-chain 3-hydroxy fatty acid (mc-3-OH-FA) perception, in the plant Arabidopsis thaliana . HAA sensing induces canonical immune signaling and local resistance to plant pathogenic Pseudomonas infection. By contrast, RLs trigger an atypical immune response and resistance to Pseudomonas infection independent of LORE. Thus, the glycosyl moieties of RLs, although abolishing sensing by LORE, do not impair their ability to trigger plant defense. Moreover, our results show that the immune response triggered by RLs is affected by the sphingolipid composition of the plasma membrane. In conclusion, RLs and their precursors released by bacteria can both be perceived by plants but through distinct mechanisms.
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Dates et versions

hal-03356415 , version 1 (28-09-2021)

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Romain Schellenberger, Jérôme Crouzet, Arvin Nickzad, Lin-Jie Shu, Alexander Kutschera, et al.. Bacterial rhamnolipids and their 3-hydroxyalkanoate precursors activate Arabidopsis innate immunity through two independent mechanisms. Proceedings of the National Academy of Sciences of the United States of America, 2021, 118 (39), pp.e2101366118. ⟨10.1073/pnas.2101366118⟩. ⟨hal-03356415⟩
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