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Article Dans Une Revue Analytical and Bioanalytical Chemistry Année : 2022

Discrimination of deletion to point cytokine mutants based on an array of cross-reactive receptors mimicking protein recognition by heparan sulfate

Hugues Lortat-Jacob

Résumé

Differential sensing of proteins based on cross-reactive arrays and pattern recognition is a promising technique for the detection and identification of proteins. In this study, a rational biomimetic strategy has been used to prepare sensing materials capable of discriminating structurally similar proteins, such as deletion and point mutants of a cytokine, by mimicking the biological properties of heparan sulfate (HS). Using the self-assembly of two disaccharides, lactose and sulfated lactose at various ratios on the surface of a chip, an array of combinatorial cross-reactive receptors has been prepared. Coupling with surface plasmon resonance imaging (SPRi), the obtained cross-reactive array is very efficient for protein sensing. It is able to detect HS binding proteins (HSbps) such as IFNγ at nanomolar concentrations. Moreover, such a system is capable of discriminating between IFNγ and its mutants with good selectivity.
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Dates et versions

hal-03340313 , version 1 (13-10-2021)

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Maria Genua, Laurie-Amandine Garçon, Yulia Sergeeva, Els Saesen, Benjamin Musnier, et al.. Discrimination of deletion to point cytokine mutants based on an array of cross-reactive receptors mimicking protein recognition by heparan sulfate. Analytical and Bioanalytical Chemistry, 2022, 414, pp.551-559. ⟨10.1007/s00216-021-03516-z⟩. ⟨hal-03340313⟩
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