A simple, sensitive and quantitative FACS-based test for SARS-CoV-2 serology in humans and animals - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2021

A simple, sensitive and quantitative FACS-based test for SARS-CoV-2 serology in humans and animals

Agnès Maurel Ribes
  • Fonction : Auteur
Pierre Bessière
  • Fonction : Auteur
Eloïse Joly Featherstone
  • Fonction : Auteur
Timothée Bruel
Remy Robinot
  • Fonction : Auteur
Olivier Schwartz

Résumé

Serological tests are important for understanding the physiopathology and following the evolution of the Covid-19 pandemic. Assays based on flow cytometry (FACS) of tissue culture cells expressing the spike (S) protein of SARS-CoV-2 have repeatedly proven to perform slightly better than the plate-based assays ELISA and CLIA (chemiluminescent immuno-assay), and markedly better than lateral flow immuno-assays (LFIA). Here, we describe an optimized and very simple FACS assay based on staining a mix of two Jurkat cell lines, expressing either high levels of the S protein (Jurkat-S) or a fluorescent protein (Jurkat-R expressing m-Cherry, or Jurkat-G, expressing GFP, which serve as an internal negative control). We show that the Jurkat-S&R-flow test has a much broader dynamic range than a commercial ELISA test and performs at least as well in terms of sensitivity and specificity. Also, it is more sensitive and quantitative than the hemagglutination-based test HAT, which we described recently. The Jurkat-flow test requires only a few microliters of blood; thus, it can be used to quantify various Ig isotypes in capillary blood collected from a finger prick. It can be used also to evaluate serological responses in mice, hamsters, cats and dogs. FACS tests offer a very attractive solution for laboratories with access to tissue culture and flow cytometry who want to monitor serological responses in humans or in animals, and how these relate to susceptibility to infection, or re-infection, by the virus, and to protection against Covid-19.
Fichier principal
Vignette du fichier
Jurkat-S&R-Flow paper for HAL 220113.pdf (8.7 Mo) Télécharger le fichier
Tables of data for Jurkat-S&R-flow paper_0609.xlsx (45.97 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03338518 , version 1 (08-09-2021)
hal-03338518 , version 2 (13-01-2022)

Identifiants

Citer

Agnès Maurel Ribes, Pierre Bessière, Jean Charles Guéry, Eloïse Joly Featherstone, Timothée Bruel, et al.. A simple, sensitive and quantitative FACS-based test for SARS-CoV-2 serology in humans and animals. 2021. ⟨hal-03338518v2⟩
133 Consultations
30 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More